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Dissertation
Detection of cells, proteins, and DNA using tapered fiber-optic biosensors (TFOBS)
Doctor of Philosophy (Ph.D.), Drexel University
May 2007
DOI:
https://doi.org/10.17918/etd-3471
Abstract
Tapered Fiber Optic Biosensors (TFOBS) have a wide variety of applications including medical diagnostics, drug screening, pathogen detection, and environmental contaminants detection. Over the past four years, TFOBS were developed in our laboratory to detect cells, proteins, and DNA. In particular, the use of TFOBS as intensity-based label-free sensors, long wavelengths for detection, and the incorporation of sample flow into detection are distinct achievements of this project. TFOBS were fabricated with using a fusion splicer and housed in a plexiglass fiber holders or flow cells. Non-specific response of the TFOBS was measured byrecording the transmission due to the presence of E.coli JM101 cells and glucose solutions. For detection experiments, antibodies or thiolated DNA probes wereimmobilized on the TFOBS surface using silane chemistry or gold coating. Detection was carried out by recording the transmission while antibody or DNA probe-immobilized TFOBS were exposed to various concentrations of analytes. Some of the highlight results of this study include the following: 1) The nonspecific measurement of 100 to 7 million E.coli JM101 cells/mL was possible in thevisible range; 2) at 1310 and 1550 nm, the TFOBS were sensitive to 0.01 to 0.1 g/mL of glucose, which gives a sensitivity in terms of dB/RI; 3) antibody-immobilized TFOBS was able to detect down to 70 E.coli O157:H7 cells/mL in the visible range; 4) antibody-immobilized TFOBS detected down to 10 fg/mL of BSA at 1310 and 1550 nm in stagnant condition; 5) antibody-immobilized TFOBS detected BSA at 1 pg/mL in flow; 6) ss-DNA probe-immobilized TFOBS detected 1 pM target DNA in flow.
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Details
- Title
- Detection of cells, proteins, and DNA using tapered fiber-optic biosensors (TFOBS)
- Creators
- Angela Leung - DU
- Contributors
- P. Mohana Shankar (Advisor) - Drexel University (1970-)Rajakkannu Mutharasan (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Chemical (and Biological) Engineering [Historical]; College of Engineering (1970-2026); Drexel University
- Other Identifier
- 3471; 991014632221604721