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Dissertation
Development of microfermenter chip
Doctor of Philosophy (Ph.D.), Drexel University
1997
DOI:
https://doi.org/10.17918/00008892
Abstract
Fermenters are used widely in biotechnology industry to grow cells and obtain information on their metabolic behavior, which is essential for developing industrial processes. To obtain such data in a short time, it is advantageous to run many fermenters concurrently. However, the capital and the operating costs become very high. The microfermenter chip developed in this work is intended to collect process data just as in a regular fermenter but at a much reduced cost. The microfermenter chip looks just like any other integrated circuit chip except that the chip has one or more well-instrumented micro-scale fermenters on it. Each fermenter is a bit smaller than a dime in size and has micro-scale sensors for measuring dissolved oxygen, pH, glucose, and cell density. These sensors are made from thin films of metals and dielectric materials, and are located at the bottom of the microfermenter. The sensors of the microfermenter were characterized in terms of measurement range, linearity, and stability. The micro pH sensor showed a linear response in the pH range of 2 to 10 with a slope of approximately -50 mV/pH at 20[degrees]C in an air saturated medium. The micro pH sensor showed good long term stability. The sensitivity of the pH sensor decreased as the oxygen level in the medium decreased. The micro DO sensor showed a linear response to partial pressure of oxygen. The micro glucose sensor was designed to operate in a kinetic regime, and the sensor showed a non-linear response. Cell density was measured by a micro interdigitated capacitance sensor which showed a linear response to cell concentrations. The capacitance was measured with varying depth of yeast solution from 0.5 mm to 1.5 mm. The capacitance was reduced with increasing the depth of yeast solution. It was demonstrated that the micro sensors were capable of measuring process variable in a batch fermentation.
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Details
- Title
- Development of microfermenter chip
- Creators
- Jong Woo Kim
- Contributors
- Young Hee Lee (Advisor) - Drexel University, Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- x, 141 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Materials (Science and) Engineering (Metallurgical Engineering) [Historical]; College of Engineering (1970-2026); Drexel University
- Other Identifier
- 991021888852604721