Files and links (1)
Janik_Joseph_19834.67 MB
PDF Restricted Access, VIEWABLE UPON REQUEST: contact archives@drexel.edu
Dissertation
Estrogen receptors in Gladiolus primulinus, and Studies on estrogen stimulation of reproductive cells in Sprague-Dawley rats
Doctor of Philosophy (Ph.D.), Drexel University
Jul 1983
DOI:
https://doi.org/10.17918/00009203
Abstract
An endogenous receptor for 17 (beta)-estradiol was characterized from the ovules of Gladiolus primulinus. This protein "translocates" from the cytoplasm to the nucleus upon activation by 17 (beta)-estradiol. The activated receptor has a sedimentation coefficient of 4.7 (+OR-) 0.1, and association constants with total specific binding capacities in the cytoplasm of 8.9 x 10('8) M('-1) and 16.2 x 10('-4) picomoles of estradiol per mg of tissue; and in the nucleus of 9.1 x 10('8) M('-1) and 22.6 x 10('-4) picomoles of estradiol per mg of tissue. Estradiol, estriol and diethyl-stilbestrol bind to this receptor while testosterone and progesterone essentially do not. This receptor was not detected in the anthers. Stimulation by 17 (beta)-estradiol induced a quantitative synthesis of proteins (receptors) which specifically bind progesterone in the uterine and ovarian cells of Sprague-Dawley rats. Both ovarectomized and intact animals had a similiar response. In the estrogen primed uterus of Sprague-Dawley rats, a receptor for epidermal growth factor (EGF) was present on myometrial cells but not on epithelial or stromal cells. The EGF receptor has an association constant of 6.4 x 10('9) M('-1) with a total specific binding capacity of 56 picomoles of EGF per mg of uterus. Preliminary evidence suggest the existence of a similiar EGF receptor on Human adenocarcinoma cells (HEC-59).
Metrics
10 Record Views
Details
- Title
- Estrogen receptors in Gladiolus primulinus, and Studies on estrogen stimulation of reproductive cells in Sprague-Dawley rats
- Creators
- Joseph R. Janik
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- ix, 104 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- College of Science (1970-1990); Drexel University
- Other Identifier
- 991021888833504721