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Dissertation
Immunogenetic studies on the mechanisms of host resistance against experimental vaginal candidiasis
Doctor of Philosophy (Ph.D.), Allegheny University of the Health Sciences
Oct 1998
DOI:
https://doi.org/10.17918/00007721
Abstract
The roles that peripheral and local cell mediated immunity play in protection from vaginal infections remain unknown. The goal of the studies presented in this thesis is to identify genetic loci and the functions regulated by these loci that are important in determining resistance to vaginal infections with Candida albicans after peripheral immunization. Both BALB/c and C57BL/6 (B6) strains of mice mount a protective immune response against vaginal candidiasis after an intravaginal immunization. In contrast, the protective effects of peripheral immunization against a subsequent vaginal infection appear to be strain-dependent. Significant protection against vaginal candidiasis after a subcutaneous immunization with C. albicans extract was achieved in BALB/c and (BALB/c X C57BL/6)F1 mice but not in B6 mice. Protection from vaginal challenge after peripheral immunization in BALB/c mice is mediated by a secondary immune response associated with a CD4, but not CD8 population of T cells. The ability of F1-derived immune cells to transfer protection to naive parental strains was obvious in BALB/c recipient mice, but much less apparent in B6 recipient mice. Cells derived from F1 immunized mice were able to be traced into the vaginal mucosa of BALB/c mice but not B6 mice. These results are highly suggestive that the microenvironment of the B6 mouse is at least in part responsible for the susceptible phenotype. Linkage analysis using CXB recombinant inbred strains, and (BALB/c X C57BL/6) X C57BL/6 backcross mice revealed that the intercellular adhesion molecule-1 (ICAM-1) and the Icam-1 related sequence 1, are strong candidate genes for controlling resistance to vaginal candidiasis after peripheral immunization. Thus, it appears that the ability of cells to bind to vaginal endothelial cells may play an important role in protection mediated by peripheral immunization. These results may contribute to the understanding of host genetic factors controlling the immune response to vaginal infections.
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Details
- Title
- Immunogenetic studies on the mechanisms of host resistance against experimental vaginal candidiasis
- Creators
- Rocio de Lourdes Mulero-Marchese
- Contributors
- Kenneth J. Blank (Advisor) - Drexel University, Allegheny University of the Health Sciences (1996-1998)
- Awarding Institution
- Allegheny University of the Health Sciences
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Allegheny University of the Health Sciences; Philadelphia, Pennsylvania
- Number of pages
- x, 146 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Allegheny University of the Health Sciences (1996-1998); School of Medicine (1996-1998); Pathology (and Laboratory Medicine) [Historical]
- Other Identifier
- 991021888897204721