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Dissertation
Lipoproteins and exchangeable apolipoproteins: kinetics and mechanism of apolipoprotein transfer
Doctor of Philosophy (Ph.D.), Allegheny University of the Health Sciences
Aug 1997
DOI:
https://doi.org/10.17918/00008273
Abstract
Although the importance of apolipoprotein transfer in lipoprotein remodeling was recognized, the mechanism of apolipoprotein transfer between lipoprotein species was not defined. The goal of this research was to define the mechanism of exchange or transfer of apolipoproteins between lipoprotein particles. The approach was to monitor the transfer of a single apolipoprotein from donor to acceptor lipoprotein particles. The following aims were addressed. Aim 1: To delineate the kinetics and mechanism of passive exchange/transfer of apoCIII molecules between human VLDL and HDL3. Aim 2: To determine the impact of apolipoprotein structure on the passive exchange/transfer of apolipoproteins between VLDL and HDL3. Aim 3: To delineate the kinetics and mechanism of passive exchange/transfer of apoC molecules between human LDL and HDL3. Kinetic studies confirmed an aqueous diffusion mechanism mediated the transfer of apoCIII molecules between VLDL and HDL3 particles. Apolipoprotein net charge, as conferred by terminal sialic acid residues, did not impact the transfer of apoCIII from donor VLDL to acceptor HDL3 particles. ApoE transfer also was suggested to proceed by an aqueous diffusion mechanism. Comparison of kinetic parameters generated with apoC and apoE suggested that apolipoprotein conformation played a role in the transfer of apolipoproteins. Kinetic studies also suggested that the highly curved surface and surface crowding on apoB-containing lipoprotein particles affected the ability of exchangeable apolipoprotein to equilibrate into and transfer from the particle.
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Details
- Title
- Lipoproteins and exchangeable apolipoproteins
- Creators
- Kathleen Erin Boyle
- Contributors
- Sissel Lund-Katz (Advisor) - Drexel University, Allegheny University of the Health Sciences (1996-1998)
- Awarding Institution
- Allegheny University of the Health Sciences
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Allegheny University of the Health Sciences; Philadelphia, Pennsylvania
- Number of pages
- 288 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Biochemistry [Historical]; Allegheny University of the Health Sciences (1996-1998); School of Medicine (1996-1998)
- Other Identifier
- 991021888914304721