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Dissertation
Studies on selected genotoxic and toxic properties of piperine
Doctor of Philosophy (Ph.D.), Drexel University
1984
DOI:
https://doi.org/10.17918/00009557
Abstract
Piperine, a natural component of black pepper, was studied to determine some of its toxicological properties. The study was conducted by examining its mutagenicity in S. typhimurium both in vitro and in vivo, observing the effect of piperine on the survival of various DNA repair deficient S. typhimurium and E. coli bacteria, determining the LD(,50) in mice and rats to see if species differences in metabolism or sensitivity exist, investigating the induction of hepatic mono-oxygenases during piperine administration to the rat via the diet, and finally a chronic toxicity study looking for possible carcinogenic properties. Piperine showed a weak mutagenicity in base-pair substitution detecting bacteria with preincubation of piperine with murine liver S9 homogenates and microsomes. Induction of P-450 enzymes by phenobarbital enhanced piperine's mutagenic response. In vivo host/mediated assay in the mouse corroborated the in vitro findings and pointed up the possibility of another metabolic pattern, one resulting in a frameshift mutagen. The mutagenicity assays suggested a difference in metabolism of piperine by rat and mouse. Determination of the LD(,50) showed the mouse to be much more sensitive to piperine related lethality. Enhanced P-450 metabolism observed as a result of piperine consumption indicated that piperine is a hepatic enzyme inducer in the rat. Long-term piperine consumption revealed no histologic differences between experimental and controls except for one of four rats developing forestomach neoplasia. Body weight gains and food consumption patterns were altered using either a low or high piperine content in the diet.
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Details
- Title
- Studies on selected genotoxic and toxic properties of piperine
- Creators
- Scott Kindell Shore
- Contributors
- Robert L. Buchanan (Advisor) - Drexel University, Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xi, 156 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Environmental Studies Institute (1970-1997); Drexel University
- Other Identifier
- 991021888766104721