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Dissertation
Surface characteristics which influence cell interaction with PMMA intraocular lenses
Doctor of Philosophy (Ph.D.), Drexel University
1994
DOI:
https://doi.org/10.17918/00007895
Abstract
A radio-frequency (RF) plasma/glow discharge surface polymerization process which significantly decreases the amount of physical cell outgrowth on poly(methyl methacrylate) (PMMA) squares has been studied. A statistically significant reduction in cell attachment, adhesion and spreading of bovine lens epithelial or rabbit corneal endothelial cells onto RF-plasma modified PMMA intraocular lenses (IOLs) compared to standard, commercial (as received) PMMA intraocular lenses (IOLs) in cell culture has also been shown. The surface modification process utilizes an argon RF-plasma "non-polymer forming" surface activation as well as a RF-plasma polymerization surfacing technique. The RF-plasma surface polymerization process decreases the amount of cell outgrowth and cell attachment, adhesion and spreading on the RF-plasma modified PMMA substrates. The final RF-plasma modified PMMA substrates are also microscopically smoother and/or have improved surface energy characteristics as compared to the unmodified PMMA squares and unmodified PMMA IOLs manufactured using the currently available mechanical, tumble polishing techniques. The RF-plasma surface polymerization process can generate the same chemical surface composition as the PMMA substrates, using RF-plasma polymerized methyl methacrylate, or can have the potential of changing the surface chemistry on the PMMA substrates using various RF-plasma polymerized hydrophilic or hydrophobic organic monomers. The irregular, semi-smooth hydrophobic PMMA substrates were surface grafted and exhibited an ultrasmooth, pinhole free surface with the same surface chemistry and energy using special RF-plasma polymerization techniques by covalenting bonding RF-plasma polymerized MMA. The PMMA substrates also exhibited a relatively smoother surface finish combined with different surface chemistry and energy characteristics by covalently bonding RF-plasma polymerized hydrophilic acrylic acid or ethylenediamine and hydrophobic hexamethyldisiloxane organic monomers. This research has shown that the modified surface of the RF-plasma polymerized PMMA IOLs showed a statistically significant decrease in the amount of cell attachment, adhesion and spreading as compared with extensive cell attachment, adhesion, and spreading on the unmodified PMMA IOLs. Therefore, due to the reduced amount of cell activity, secondary opacification, pseudophakic precipitates, and synechiae, are less likely to occur on the RF-plasma modified IOLs after surgical implantation.
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Details
- Title
- Surface characteristics which influence cell interaction with PMMA intraocular lenses
- Creators
- David Michael Kitinoja
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xiv, 116 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Drexel University
- Other Identifier
- 991021888906204721