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Dissertation
Theoretical and experimental approaches to control blood vessel growth into tissue engineered scaffolds
Doctor of Philosophy (Ph.D.), Drexel University
Jun 2007
DOI:
https://doi.org/10.17918/etd-1794
Abstract
The field of tissue engineering s to regenerate natural tissues and create new tissues using combinations of living cells, biomaterials and signaling molecules. One of the important requirements for biomaterials is that they allow for development of healthy, internal capillary networks. The objective of this project was to explore the underlying mechanisms that control vascular growth into porous biomaterials through a co-operation between experimentation and mathematical modeling. We developed a a discrete probabilistic mathematical model of cell migration and vascular growth into porous biomaterials. Some of the highlight results of this study included the following: (i) transience in chemoattractant secretion profiles determined the effectiveness of cell motility and cell-to-cell signaling, (ii) tuning the pore surface reactivity to angiogenic factors offered a possible mechanism to hasten the speed of cell migration and tissue ingrowth, and (iii) optimal release durations of an angiogenic growth factor induced deeper vascular growth into the scaffold. The experimental part of this project aimed at exploring the variables that influence the extent of vascular growth in 3D porous poly(1actide-co-glycolide) (PLAGA) sintered microsphere scaffolds. In addition, we designed a combined gene-therapy/cell- transplantation strategy to induce vascular ingrowth. Some of the highlight results of this work were: (i) PLAGA scaffolds supported the growth and biological functions of endothelial cells and adipose derived stromal cells (ADSCs), (ii) vascular endothelial growth factor (VEGF) transfected ADSCs secreted biologically active VEGF on scaffolds, (iii) in-vivo implantation of PLAGA scaffolds containing transfected ADSCs and endothelial cells showed a significantly improved vascular ingrowth.
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Details
- Title
- Theoretical and experimental approaches to control blood vessel growth into tissue engineered scaffolds
- Creators
- Ehsan Jabbarzadeh - DU
- Contributors
- Cameron F. Abrams (Advisor) - Drexel University (1970-)Cato T. Laurencin (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Chemical and Biological Engineering; College of Engineering; Drexel University
- Other Identifier
- 1794; 991014632254804721