Encyclopedia entry
Human liver on a chip: engineering liver sinusoid on a chip
MicroTAS, International Conference on Miniaturized Systems for Chemistry and Life Sciences, 19, pp.756-758
01 Jan 2016
Abstract
We developed a liver on a chip system to mimic the liver sinusoid. Primary human hepatocytes (PHHs) and immortalized bovine aortic endothelial cells (BAECs) were co-cultured on the opposite sides of a porous membrane in a dual microfluidic channel. PHHs co-cultured with BAECs retained their morphology for 40 days. The Hepatitis B core antigen (HBcAg) of PHHs infected with Hepatitus B virus (HBV) was detected through immunofluorescence assay. HBV replication, as measured by the presence of cell-secreted HBV-DNA, was successfully detected. We believe that our human liver on a chip could find numerous applications in liver-related research and drug development.
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Details
- Title
- Human liver on a chip: engineering liver sinusoid on a chip
- Creators
- Y.B KangN DucheminS RawatJ LamontagneM BouchardM Noh
- Publication Details
- MicroTAS, International Conference on Miniaturized Systems for Chemistry and Life Sciences, 19, pp.756-758
- Conference
- MicroTAS, International Conference on Miniaturized Systems for Chemistry and Life Sciences
- Publisher
- WTI-Frankfurt-digital GmbH
- Number of pages
- 1
- Resource Type
- Encyclopedia entry
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Mechanical Engineering and Mechanics
- Identifiers
- 991019173553604721