Thesis
Control physicochemical properties of nanoparticle surfaces to modulate immune-cell recruitment to scaffolds
Master of Science (M.S.), Drexel University
Jun 2017
DOI:
https://doi.org/10.17918/etd-7802
Abstract
Although many complex tissue engineering scaffolds have been developed, the number of translated treatments remains stagnant. Successful applications of scaffolds in regenerative medicine often requires scaffolds to elicit minimal host immune response and controlled release of bioactive molecules. Nanoparticles have shown great promise in the controlled release of therapeutics to aid tissue engineering. However, nanoparticles incorporated in tissue engineering scaffolds causes extra inflammatory responses. Although the release of anti-inflammatory drugs has been shown to reduce the foreign body response to scaffolds in vivo, the drugs also interfere with immune cells that are vital for tissue regeneration. Proposed here is a nanoparticle embedded scaffold drug delivery system designed to minimize the host immune response by controlling the physiochemical properties of nanoparticles. This system embeds PLGA nanoparticles with or without encapsulated immunosuppressant drugs in the walls of alginate scaffolds. The effects of surface modification and release of dexamethasone on immune cell recruitment to scaffolds in vivo were explored. Red Blood Cell Membrane (RBCM) coating on PLGA nanoparticles showed a decrease in neutrophil recruitment as well as an increased number of CD4+ T cells in the scaffolds, which are important for tissue regeneration. Our approach is a new strategy in minimizing the induced foreign body inflammatory response of nanoparticles used for tissue regeneration.
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Details
- Title
- Control physicochemical properties of nanoparticle surfaces to modulate immune-cell recruitment to scaffolds
- Creators
- Stephen C. Bady - DU
- Contributors
- Caroline L. Schauer (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- ix, 57 pages
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- Materials (Science and) Engineering (Metallurgical Engineering) (1970-2026); College of Engineering (1970-2026); Drexel University
- Other Identifier
- 7802; 991014632839604721