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Thesis
Electrospun PLLA/SWNT nanocomposite fibril for cartilage regeneration
Master of Science (M.S.), Drexel University
Jun 2004
DOI:
https://doi.org/10.17918/etd-302
Abstract
In this study, Poly-L-Lactic acid has been used as the base polymer to fabricatean electrospun, 3-dimensional, fibrillar scaffold for cartilage regeneration. In the scaffolds, fiber diameters as low as 150nm were obtained with an average of550nm. The mechanical properties of the scaffold were improved, with a threefoldincrease in modulus, by co-electrospinning the reinforcing material (SWNT)with the polymer. The presence and alignment of SWNT in the fibers wasconfirmed by Raman spectroscopy and TEM respectively. The scaffolds wereseeded with human chondrocytes (Cell Applications Inc) and studied for a periodof nine weeks. The scaffolds containing SWNT showed no adverse effect on theviability of the cells seeded on them. The cell attachment to the scaffoldcontaining SWNT was increased by incorporating Poly-[epsilon]-L-Lysine ([epsilon]-PL) in theelectrospinning dope. In the presence of [epsilon]-PL, seeded chondrocytes tended tomaintain a guided growth along the fibrils. Morphology and viability of thechondrocytes seeded on the scaffold were confirmed by ESEM and MTT assay.
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Details
- Title
- Electrospun PLLA/SWNT nanocomposite fibril for cartilage regeneration
- Creators
- Shairali Shiva Rao - DU
- Contributors
- Frank K. Ko (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems (1997-2026); Drexel University
- Other Identifier
- 302; 991014632520604721