Thesis
HSV-1 infection in vaginal epithelial cells is inhibited by application of non-thermal plasma-conditioned medium
Master of Science (M.S.), Drexel University
Jul 2024
DOI:
https://doi.org/10.17918/00010590
Abstract
Genital infection by herpes simplex virus type 1 (HSV-1) is characterized by a cycle of symptomatic lytic infection, corresponding to the appearance of anogenital lesions, and asymptomatic latent infection. Current antiviral therapies relieve the symptoms of acute infection but do not resolve the infection. We propose the use of plasma conditioned-medium (PCM) as an antiviral agent for therapeutic use on genital HSV-1 infections. In an in vitro model of infection, we demonstrated that application of PCM to a vaginal epithelial cell line reduced viral replication in HSV-1-infected cells and lowered susceptibility of uninfected cells to subsequent HSV-1 infection. The antiviral effects of PCM were replicated in polarized epithelial cells in a transwell culture system. Medium collected from the basolateral chamber also exhibited antiviral activity, illustrating indirect effects of PCM on HSV-1 replication. Our findings suggest that PCM is a promising antiviral agent that will counter the viral pathogenesis in HSV-1 genital lesions by limiting active lytic infection in infected epithelial cells and preventing the dissemination of the virus to nearby uninfected cells.
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Details
- Title
- HSV-1 infection in vaginal epithelial cells is inhibited by application of non-thermal plasma-conditioned medium
- Creators
- Caroline Corcoran
- Contributors
- Stephen R. Jennings (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xxi pages
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- Microbiology and Immunology; College of Medicine; Drexel University
- Other Identifier
- 991021902013904721