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Identifying protein partners and binding sites on the lncRNA SChLAP1
Thesis

Identifying protein partners and binding sites on the lncRNA SChLAP1

Christopher Yamamoto Markgraf
Master of Science (M.S.), Drexel University
Aug 2024
DOI:
https://doi.org/10.17918/00010490
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Markgraf_Christopher_20241.45 MB
PDF Embargoed Access, Embargo ends: 30 Sep 2026

Abstract

lncRNA Protein binding sites SDA TREX Cytology
Prostate cancer is one of the leading causes of cancer in males, with over 280,000 projected diagnoses in 2023 and over 34,000 deaths. Despite recent progress in therapeutic interventions, metastatic prostate cancer has a 5-year survival rate of only 32%. Identifying markers and mechanisms that lead to aggressive prostate cancer is of immense importance. In recent years, long non-coding RNAs (lncRNAs), RNA molecules longer than 200nts with no coding potential, have evolved as promising biomarkers and potential targets for cancer. One such example is the lncRNA SChLAP1, an indicator of high-risk prostate cancer. Independent studies have shown that SChLAP1 is aberrantly expressed in aggressive prostate cancer and promotes tumor progression. However, the molecular mechanism behind SChLAP1's function remains unknown. LncRNAs often achieve their function through interaction with RNA-binding proteins (RBPs). RBPs have been widely characterized in cancer to be dysregulated, leading to disease progression. This thesis aims to identify specific proteins that bind SChLAP1 and their binding sites on SChLAP1. The results will guide future research into the molecular mechanism of SChLAP1, followed by cellular and in vivo assays, to gain a deeper understanding of how their interactions influence metastatic prostate cancer. Ultimately, this work may allow for the development of novel therapeutic avenues to treat metastatic prostate cancer.

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