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Thesis
Investigating the role of SND1 in Glioblastoma using RNA-Seq
Master of Science (M.S.), Drexel University
Jul 2024
DOI:
https://doi.org/10.17918/00010487
Abstract
Glioblastoma (GBM) is the most aggressive and lethal form of primary brain tumor, known for its rapid growth and high resistance to conventional therapies. Despite extensive research, the prognosis for GBM patients remains poor, with a median survival time of approximately 12 to 18 months following diagnosis. Recent studies have identified the RNA-binding protein Staphylococcal Nuclease and Tudor Domain Containing 1 (SND1) as key players in the pathogenesis of GBM. SND1 is significantly overexpressed in primary human gliomas, and its expression levels have been found to correlate closely with tumor grade, suggesting a potential role in tumor progression and malignancy. However, the specific molecular mechanism through which SND1 regulates Glioblastoma remains poorly understood. This study aimed to investigate the functional significance of SND1 in glioblastoma by knocking down its expression in U251 cells and examining the resultant effects. We utilized RNA sequencing to analyze the global changes in gene expression and identify the pathways affected by SND1 knockdown. Expression of 277 genes, including MCAM, MMP3, and TGF-α, were significantly altered upon SND1 gene silencing. The Activating Transcription Factor (ATF) binding pathway was significantly downregulated upon SND1 knockdown, highlighting its importance in regulating gene expression. This study highlights the critical role of SND1 in Glioblastoma, revealing its impact on the expression of key genes and pathways involved in tumor progression. This research may contribute to the developing SND1 as a promising therapeutic target and treating GBM.
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Details
- Title
- Investigating the role of SND1 in Glioblastoma using RNA-Seq
- Creators
- Tisha Kalpesh Desai
- Contributors
- Srinivas Somarowthu (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- vii, 59 pages
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; College of Medicine; Drexel University
- Other Identifier
- 991021895514904721