Thesis
Isolation, purification, and characterization of cell membrane vesicles
Master of Science (M.S.), Drexel University
Jun 2020
DOI:
https://doi.org/10.17918/00000181
Abstract
Biologically sourced nanomaterials have been actively explored in order to create solutions to long held difficulties in the biomedical field including drug delivery, cell targeting, intercellular communication, and removing of toxin. Although cell membrane-derived vesicles have been successfully applied to address several important medical needs in bench research, the methods of formation, purification, stabilization, and characterization of these particles requires further optimization and exploration. To ensure their function, it is important to control vesicle parameters, including vesicle size, stability over time, and purity. Proposed herein are methods and experiments created to isolate, purify, and characterize cell membrane-derived vesicles in a controlled manner. In order to exhibit control over these parameters, the central aims of this project are to create membrane vesicles approximately 100 nm in size, with high degrees of purity, through fabrication methods that are efficient, robust, and effective. This work will result in information that will facilitate the rapid growth of research into membranous nanoparticles, ultimately resulting in biomedical solutions to a variety of medical needs.
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Details
- Title
- Isolation, purification, and characterization of cell membrane vesicles
- Creators
- Kevin Wu
- Contributors
- Hao Cheng (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- x, 43 pages
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- Materials (Science and) Engineering (Metallurgical Engineering) (1970-2026); College of Engineering (1970-2026); Drexel University
- Other Identifier
- 991014695141104721