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PDFOpen Access (License Unspecified), Open Access
Thesis
Novel therapeutic intervention for myocardial infarction in large animal model
Master of Science (M.S.), Drexel University
Dec 2017
DOI:
https://doi.org/10.17918/D8B36T
Abstract
Heart failure (HF) represents an enormous clinical problem that imposes a significant burden both to society and survivors. During a myocardial infarction, cardiomyocytes are damaged due to a lack of oxygen to the left ventricle and as a result these cells die. The dead cells are then replaced by fibrotic scar tissue that inhibits the heart from properly pumping blood to the rest of the heart. The current treatments of HF do not reverse the damage that the left ventricle experiences and as a result many people experience recurrent heart failure and sometime cardiac arrest. The field of cellular reprogramming investigates the process of direct reprogramming of endogenous cardiac fibroblasts to cardiomyocytes as a novel approach to help the heart to pump blood properly after a myocardial infarction. In this project, we used a biodegradable hyaluronic acid (HA) hydrogel to encapsulate Adeno-associated virus (AAV) 9 engineered to deliver the reporter gene green fluorescent protein (GFP) or five transcription factors (G: Gata4, M: Mef2c, T: Tbx5, M: Mesp1and M: Myocardin) in pigs' heart post-myocardial. Our method represents a unique approach to transfect cardiomyocyte cells in vivo post-myocardial infarct in pig. This study also demonstrates a cardiac functional improvement with the injection of hydrogel gel, AAV9, and selected transcription factors in the compromised area of the left ventricle. This novel method reduced the scar tissue in the compromised area of the heart compared to control.
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Details
- Title
- Novel therapeutic intervention for myocardial infarction in large animal model
- Creators
- Maria Mercedes - DU
- Contributors
- Andres Kriete (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- 34 pages
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems (1997-2026); Drexel University
- Other Identifier
- 7694; 991014632729304721