Files and links (1)
PDFOpen Access (License Unspecified), Open Access
Thesis
Super-porous PVA hydrogels with controlled release of IGF-1 for cartilage tissue engineering
Master of Science (M.S.), Drexel University
Jun 2012
DOI:
https://doi.org/10.17918/etd-3899
Abstract
Repair or regeneration of articular cartilage remains one of the most difficult challenges in orthopedic medicine. Poly(vinyl alcohol) (PVA) hydrogels have shown promise as synthetic cartilage replacement materials, especially those that allow sustained release of insulin-like growth factor 1 (IGF-1). The goal of this study was to increase the porosity of the previously designed hydrogels that had an average pore size of 60 to 100[mu]m and a porosity of 60 to 70%, while maintaining IGF-1 release in order to increase infiltration of chondrocytes from surrounding cartilage. The hydrogels were prepared using a previously established, single step, double emulsion technique based on a water-oil-water (W1/O/W2) composition. IGF-1 was encapsulated in degradable poly(lactic-co-glycolic acid) (PLGA) microparticles (W1/O) embedded in non-degradable poly(vinyl alcohol) (PVA) hydrogels (W2). The effects of processing parameters such as volume and composition of the water and oil phases were evaluated towards increasing hydrogel porosity. Hydrogels of various formulations had interconnected pores from 40 to 600um and average pore size of up to 337 ± 205[mu]m, and porosities of 76% to 84%, all of which were increases over the previous design. The controlled release system sustained release of IGF-1 for 73 days in vitro, with an initial burst on the first day of 1.82 ± 1.15[mu]g/ml. In order to study the controlled release of IGF-1 on cartilage tissue engineering in vivo, the hydrogels were seeded with chondrocytes and implanted subcutaneously into the backs of nude mice and harvested after two and five weeks in vivo. The introduction of high levels of porosity to PVA hydrogels resulted in a reduction in mechanical properties but an increase in cartilage growth within the hydrogels. This study shows that the sustained release of IGF-1 can enhance tissue formation within the pores of a non-degradable hydrogel. The increase in porosity of the IGF1-eluting hydrogels may improve implant integration with surrounding tissue.
Metrics
41 File views/ downloads
25 Record Views
Details
- Title
- Super-porous PVA hydrogels with controlled release of IGF-1 for cartilage tissue engineering
- Creators
- Claire Elizabeth Martin - DU
- Contributors
- Anthony M. Lowman (Advisor) - Drexel University (1970-)Kara L. Spiller (Advisor) - Drexel University (1970-)Guangdong Zhou (Advisor) - Drexel University (1970-)Wei Liu (Advisor) - Drexel University (1970-)
- Awarding Institution
- Drexel University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems (1997-2026); Drexel University
- Other Identifier
- 3899; 991014632063104721