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Sydnocarb as a candidate for cocaine addiction treatment
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Sydnocarb as a candidate for cocaine addiction treatment

Hanming Zeng
Master of Science (M.S.), Drexel University
Aug 2021
DOI:
https://doi.org/10.17918/00000854
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Abstract

Cocaine abuse--Treatment
Cocaine addiction treatment is still a great challenge, while dopamine transporter (DAT) remains a target for addiction treatment development. As we have found a molecule, KM822, binding to a novel allosteric site in DAT and could decrease the affinity of cocaine, we study a psychostimulant, sydnocarb, that could also bind to the allosteric sites and showed treatment potential in our previous in vitro study. Thus, in this paper, we tried to find in vivo evidence to support the hypothesis of sydnocarb that could influence the addiction-related behavior of cocaine. We adopt a condition place preference (CPP) model in rats to study whether sydnocarb could cause CPP or influence the CPP elicited by cocaine, which may be an indicator of rewarding. Our study has demonstrated that 1mg/kg and 5mg/kg of sydnocarb could not cause the CPP effect on its own. However, 1mg/kg pretreatment could neither inhibit the induction of CPP by cocaine and nor reduce the occurrence of CPP reinstatement. We also conduct a cFos immunohistological detection on these rats to compare the differences caused by sydnocarb pretreatment and find that there is no significant difference in cFos immunopositive cells expression related to 1mg/kg sydnocarb pretreatment in the induction session. Overall, our study demonstrates that sydnocarb is not capable of blocking the CPP induction and relapse on rats in a very low dose, and a higher dose could be safe to conduct for the future for a further understanding of this molecule.

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