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Thesis
The effect of oligomeric derivatives of prostaglandin B₁ on F₁F₀ATPase and Ca²⁺-Mg²⁺ ATPase
Master of Science (M.S.), Hahnemann University
Jun 1986
DOI:
https://doi.org/10.17918/00006699
Abstract
Oligomers of 13-dehydroPGBj and 16,16’-dimethyM5-dehydroPGBj containing from two to four monomers have been synthesized and characterized by Dr. George Nelson, Saint Joseph’s University, Philadelphia, PA. The oligomeric derivatives have some activites in common with PGBj, a mixture of higher oligomers of unknown structure. The purpose of the investigation was to determine the effects of the defined oligomers on the mitochondrial FjFQ-ATPase, whose activity is required for the maintenance of mitochondrial integrity, and the Ca^'-Mg^* ATPase of the plasma membrane, whose activity is required for the maintenance of low levels of intracellular Ca^ + The monomers and dimers of the 15-dehydroPGBj and 16.16’-dimethyl-15-dehydroPGBj exerted little effect on the FjFfl-ATPase whereas trimersand tetramers of both series inhibited the FjFq-ATPase activity as measured in submitochondrial particles. The trimers and tetramers of both series exhibited a maximum attainable inhibition of approximately 33% and 30%, respectively, at a concentration of 0.37 pM (10 nmol oligomer/mg submitochondrial particles); Kreutter and Devlin (1983) demonstrated 40% inhibition of the F|Fq-ATPase by PGBj at concentrations up to 12.3 pM 13-dehydroPGBj or 16,16-dimethyl-15-dehydroPGBj trimer also inhibited the uncoupler-stimulated ATPase activity; concentrations up to 0 37 pM resulted in a maximum attainable inhibition of 41%; PGBZ exhibited a maximum attainable inhibition of uncoupler-stimulated ATPase activity of 69% at a concentration of 5-46 MM (Kreutter and Devlin, 1983) Knudsen and Devlin (unpublished results, this laboratory) reported an inhibition of uncoupler-stimulated respiration in mitochondria by the trimers and tetramers of both series, suggesting that the active oligomers interfere with the mechanism of uncoupler-stimulated activities
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Details
- Title
- The effect of oligomeric derivatives of prostaglandin B₁ on F₁F₀ATPase and Ca²⁺-Mg²⁺ ATPase
- Creators
- Rosita S. Krupinski
- Awarding Institution
- Hahnemann University
- Degree Awarded
- Master of Science (M.S.)
- Publisher
- Hahnemann University; Philadelphia, Pennsylvania
- Number of pages
- ix, 65 pages, [15] pages of diagrams
- Resource Type
- Thesis
- Language
- English
- Academic Unit
- School of Medicine (1982-1993); Hahnemann University (1982-1993); Biochemistry [Historical]
- Other Identifier
- 991021888067604721