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10-minute assay for detecting Escherichia coli O157:H7 in ground beef samples using piezoelectric-excited millimeter-size cantilever sensors
Journal article   Open access   Peer reviewed

10-minute assay for detecting Escherichia coli O157:H7 in ground beef samples using piezoelectric-excited millimeter-size cantilever sensors

David Maraldo and Raj Mutharasan
Journal of food protection, v 70(7), pp 1670-1677
Jul 2007
DOI: https://doi.org/10.4315/0362-028X-70.7.1670
PMID: 17685341
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://doi.org/10.4315/0362-028x-70.7.1670View
Published, Version of Record (VoR)Open Access (Publisher-Specific),  Open

Abstract

Animals Biosensing Techniques - instrumentation Biosensing Techniques - methods Cattle Colony Count, Microbial - methods Consumer Product Safety Escherichia coli O157 - isolation & purification Food Contamination - analysis Food Microbiology Humans Meat Products - microbiology Time Factors
We detected Escherichia coli O157:H7 (EC) at approximately 10 cells per ml in spiked ground beef samples in 10 min using piezoelectric-excited millimeter-size cantilever (PEMC) sensors. The composite PEMC sensors have a sensing area of 2 mm2 and are prepared by immobilizing a polyclonal antibody specific to EC on the sensing surface. Ground beef (2.5 g) was spiked with EC at 10 to 10,000 cells per ml in phosphate-buffered saline (PBS). One milliliter of supernatant was removed from the blended samples and used to perform the detection experiments. The total resonant frequency change obtained for the inoculated samples was 138 +/- 9, 735 +/- 23, 2,603 +/- 51, and 7,184 +/- 606 Hz, corresponding to EC concentrations of 10, 100, 1,000, and 10,000 cells per ml, respectively. EC was detected in the sample solution within the first 10 min. The responses of the sensor to positive, negative, and buffer controls were 36 +/- 6, 27 +/- 2, and 2 +/- 7 Hz, respectively. Verification of EC attachment was confirmed by low-pH buffer release (PBS-HCl, pH 2.2), microscopy, and second antibody EC binding postdetection. The results indicate that PEMC sensors can reliably detect EC at less than 10 cells per ml in 10 min without sample preparation and with label-free reagents.

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24 citations in Web of Science
30 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#3 Good Health and Well-Being

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Web of Science research areas
Biotechnology & Applied Microbiology
Food Science & Technology
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