Logo image
Back
19F-NMR and fluorescence polarization of yeast plasma membrane and isolated lipids
Journal article   Peer reviewed

19F-NMR and fluorescence polarization of yeast plasma membrane and isolated lipids

M. Esfahani, J.R. Cavanaugh, P.E. Pfeffer, D.W. Luken and T.M. Devlin
Biochemical and biophysical research communications, v 101(1), pp 306-311
1981
DOI: https://doi.org/10.1016/S0006-291X(81)80045-9
PMID: 7025840
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

16-Fluoropalmitic acid was incorporated biosynthetically into the plasma membrane of yeast and the F-NMR spectra of the intact membrane and of aqueous dispersions of membrane lipids were compared. The greater degree of resonance broadening in the intact membrane reveals a lesser degree of mobility of the acyl chains. Fluorescence polarization studies with a probe that monitors the polar environment and one that detects viscosity changes in the inner core of the bilayer indicate hindered motions of the polar head groups and acyl chains in the intact membrane as compared to liposomes prepared from membrane lipids. The observations indicate that the bulk of lipids in the yeast membrane experiences immobilization by proteins and that membrane proteins influence lipid-lipid interactions and inhibit lipid phase transition.

Metrics

6 Record Views
16 citations in Web of Science
15 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Collaboration types
Domestic collaboration
Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
Logo image