Journal article
3-Hydroxykynurenine and 3-Hydroxyanthranilic Acid Generate Hydrogen Peroxide and Promote α-Crystallin Cross-Linking by Metal Ion Reduction
Biochemistry (Easton), v 39(24), pp 7266-7275
20 Jun 2000
PMID: 10852726
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The kynurenine pathway catabolite 3-hydroxykynurenine (3HK) and redox-active metals such as copper and iron are implicated in cataractogenesis. Here we investigate the reaction of kynurenine pathway catabolites with copper and iron, as well as interactions with the major lenticular structural proteins, the α-crystallins. The o-aminophenol kynurenine catabolites 3HK and 3-hydroxyanthranilic acid (3HAA) reduced Cu(II)>Fe(III) to Cu(I) and Fe(II), respectively, whereas quinolinic acid and the nonphenolic kynurenine catabolites kynurenine and anthranilic acid did not reduce either metal. Both 3HK and 3HAA generated superoxide and hydrogen peroxide in a copper-dependent manner. In addition, 3HK and 3HAA fostered copper-dependent α-crystallin cross-linking. 3HK- or 3HAA-modifed α-crystallin showed enhanced redox activity in comparison to unmodified α-crystallin or ascorbate-modified α-crystallin. These data support the possibility that 3HK and 3HAA may be cofactors in the oxidative damage of proteins, such as α-crystallin, through interactions with redox-active metals and especially copper. These findings may have relevance for understanding cataractogenesis and other degenerative conditions in which the kynurenine pathway is activated.
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Details
- Title
- 3-Hydroxykynurenine and 3-Hydroxyanthranilic Acid Generate Hydrogen Peroxide and Promote α-Crystallin Cross-Linking by Metal Ion Reduction
- Creators
- Lee E. Goldstein - Brigham and Women's HospitalMichael C. Leopold - Brigham and Women's HospitalXudong Huang - Brigham and Women's HospitalCraig S. Atwood - Brigham and Women's HospitalAleister J. Saunders - Brigham and Women's HospitalMariana Hartshorn - Brigham and Women's HospitalJames T. Lim - Brigham and Women's HospitalKyle Y. Faget - Brigham and Women's HospitalJulien A. Muffat - Brigham and Women's HospitalRichard C. Scarpa - Brigham and Women's HospitalLeo T. Chylack - Brigham and Women's HospitalEdmond F. Bowden - Brigham and Women's HospitalRudolph E. Tanzi - Massachusetts General HospitalAshley I. Bush - Brigham and Women's Hospital
- Publication Details
- Biochemistry (Easton), v 39(24), pp 7266-7275
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 10
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000087778300027
- Scopus ID
- 2-s2.0-0034691246
- Other Identifier
- 991021448038104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology