Files and links (1)
Published, Version of Record (VoR)CC BY V4.0, Open
Journal article
3D Image Analysis of the Complete Ventricular-Subventricular Zone Stem Cell Niche Reveals Significant Vasculature Changes and Progenitor Deficits in Males Versus Females with Aging
Stem cell reports, v 16(4), pp 836-850
13 Apr 2021
PMID: 33836145
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
With age, neural stem cell (NSC) function in the adult ventricular-subventricular zone (V-SVZ) declines, reducing memory and cognitive function in males; however, the impact on females is not well understood. To obtain a global view of how age and sex impact the mouse V-SVZ, we constructed 3D montages after multiplex immunostaining, and used computer-based 3D image analysis to quantify data across the entire niche at 2, 18, and 22 months. We discovered dramatic sex differences in the aging of the V-SVZ niche vasculature, which regulates NSC activity: females showed increased diameter but decreased vessel density with age, while males showed decreased diameter and increased tortuosity and vessel density. Accompanying these vascular changes, males showed significant decline in NSC numbers, progenitor cell proliferation, and more disorganized migrating neuroblast chains with age; however, females did not. By examining the entire 3D niche, we found significant sex differences, with females being relatively spared through very old age.
[Display omitted]
•Aging impacts the mouse V-SVZ significantly more in males than females•Vessel tortuosity, narrowness, and density increase in aged males not females•Progenitor cell division and neuroblast chain organization decline in aging males•Numbers of type B stem cells decline significantly in aged males not females
To explore sex differences with age in the adult mouse neural stem cell niche, Temple and colleagues analyzed entire 3D V-SVZ whole-mounts via computer-based multiplex image analysis. Sexual dimorphism was seen with aging in the vascular plexus, a critical niche component, along with more severe decline in neural stem cell number, progenitor proliferation and neuroblast organization in males compared with females.
Metrics
Details
- Title
- 3D Image Analysis of the Complete Ventricular-Subventricular Zone Stem Cell Niche Reveals Significant Vasculature Changes and Progenitor Deficits in Males Versus Females with Aging
- Creators
- Xiuli Zhao - Neural Stem Cell InstituteYue Wang - Neural Stem Cell InstituteEric Wait - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USA; Advanced Imaging Center, Howard Hughes Medical Institute Janelia Research Campus, Ashburn, VA 20147, USA.Walt Mankowski - Department of Electrical and Computer Engineering, Drexel University, Philadelphia, PA 19104, USAChristopher S. Bjornsson - Neural Stem Cell InstituteAndrew R. Cohen - Drexel UniversityKristen L. Zuloaga - Albany Medical Center HospitalSally Temple - Neural Stem Cell Institute
- Publication Details
- Stem cell reports, v 16(4), pp 836-850
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Electrical and Computer Engineering
- Web of Science ID
- WOS:000640267100012
- Scopus ID
- 2-s2.0-85103964012
- Other Identifier
- 991019168062304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology