Journal article
3D Printing Cervical Implant Scaffolds Incorporated with Drug-Loaded Carboxylated Chitosan Microspheres for Long-Term Anti-HPV Protein Delivery
ACS biomaterials science & engineering, v 10(3), pp 1544-1553
11 Mar 2024
PMID: 38369785
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
As attempting personalized medicine, 3D-printed tissue engineering scaffolds are employed to combine with therapeutic proteins/peptides especially in the clinical treatment of infectious diseases, genetic diseases, and cancers. However, current drug-loading methods, such as immersion and encapsulation, usually lead to the burst release of the drugs. To address these issues, we proposed an integrated strategy toward the long-term controlled release of protein. In this study, patient-customized 3D scaffolds incorporated with drug-loaded microspheres were printed to realize the effective delivery of the anti-human papillomavirus (anti-HPV) protein after cervical conization in the treatment of cervical cancer. The 3D-printed scaffold could provide mechanical support to the defect site and ensure local release of the drug to avoid systemic administration. Meanwhile, microspheres serve as functional components to prevent the inactivation of proteins, as well as regulate their release period to meet the time requirement of different treatment courses. The research also utilized bovine serum albumin as a model protein to validate the feasibility of these scaffolds as a generic technology platform. The bioactivity of the released anti-HPV protein was validated using a pseudovirus model, which demonstrated that the microsphere encapsulation would not cause protein denaturation during the scaffold fabrication process. Besides, 3D-printed scaffolds incorporated with carboxylated chitosan microspheres were biocompatible and beneficial for cell attachment, which have been demonstrated by favorable cell viability and better coverage results for HeLa and HFF-1. This study highlights the great potential of scaffolds incorporated with microspheres to serve as tissue engineering candidate products with the function of effective protein delivery in a long-term controlled manner and personalized shapes for clinical trials.
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Details
- Title
- 3D Printing Cervical Implant Scaffolds Incorporated with Drug-Loaded Carboxylated Chitosan Microspheres for Long-Term Anti-HPV Protein Delivery
- Creators
- Jingyuan Ji - Tsinghua UniversityChenjia Zhao - Tsinghua UniversityChen Hua - Fudan UniversityLu Lu - Fudan UniversityYuan Pang (Corresponding Author) - Tsinghua UniversityWei Sun - Drexel University
- Publication Details
- ACS biomaterials science & engineering, v 10(3), pp 1544-1553
- Publisher
- ACS Publications
- Number of pages
- 10
- Grant note
- 3212007 / Beijing Natural Science Foundation 20220484075 / Beijing Nova Program; Beijing Municipal Science & Technology Commission 2022NSCQ-LZX0326 / Natural Science Foundation of Chongqing 2022ZLB004 / Tsinghua University Initiative Scientific Research Program; Tsinghua University 52175273; 52211540006 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) B17026 / The 111 Project; Ministry of Education, China - 111 Project
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001174519500001
- Scopus ID
- 2-s2.0-85186100322
- Other Identifier
- 991022202099204721
UN Sustainable Development Goals (SDGs)
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Source: SDGs in the Output
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Materials Science, Biomaterials