Journal article
3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
Journal of Visualized Experiments, v 2021(170)
2021
PMID: 33970133
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microcarriers are beads with a diameter of 60-250 µm and a large specific surface area, which are commonly used as carriers for large-scale cell cultures. Microcarrier culture technology has become one of the main techniques in cytological research and is commonly used in the field of large-scale cell expansion. Microcarriers have also been shown to play an increasingly important role in in vitro tissue engineering construction and clinical drug screening. Current methods for preparing microcarriers include microfluidic chips and inkjet printing, which often rely on complex flow channel design, an incompatible two-phase interface, and a fixed nozzle shape. These methods face the challenges of complex nozzle processing, inconvenient nozzle changes, and excessive extrusion forces when applied to multiple bioink. In this study, a 3D printing technique, called alternating viscous-inertial force jetting, was applied to enable the construction of hydrogel microcarriers with a diameter of 100-300 µm. Cells were subsequently seeded on microcarriers to form tissue engineering modules. Compared to existing methods, this method offers a free nozzle tip diameter, flexible nozzle switching, free control of printing parameters, and mild printing conditions for a wide range of bioactive materials.
Metrics
Details
- Title
- 3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting
- Creators
- Tiankun Liu - Tsinghua UniversityYongchun Shao - Tsinghua UniversityZitong Wang - Tsinghua UniversityYuqiu Chen - Tsinghua UniversityYuan Pang - Tsinghua UniversityDing Weng - Tsinghua UniversityWei Sun - Biomanufacturing Center, Dept. of Mechanical Engineering, Tsinghua University; Biomanufacturing and Rapid Forming Technology Key Laboratory of Beijing; Department of Mechanical Engineering, Drexel University;
- Publication Details
- Journal of Visualized Experiments, v 2021(170)
- Publisher
- MyJove Corporation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000646217000056
- Scopus ID
- 2-s2.0-85105736451
- Other Identifier
- 991019167654404721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary