Journal article
3D perfusion culture of mouse insulinoma in macro-porous scaffolds enhanced insulin production response
International journal of artificial organs, v 45(1)
Jan 2022
PMID: 33380250
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
To address the remaining issue of poor cell immobilization and insufficient mass transfer in scaffold-based tissue engineering approach for future islet transplantation, we employed a macro-porous poly-l-lactide (PLLA) scaffold immobilizing mouse insulinoma cells and studied its function toward an implantable pancreatic tissue in 7-day perfusion culture. The murine pancreatic β cells could be immobilized in the PLLA scaffold at a high density of 10
cells per cm
close to the estimated range in normal pancreas. The perfusion culture promoted the 3D cellular organization as observed with live/dead staining and histological staining. The insulin production was significantly enhanced in comparison with static 2D culture and 3D rotational suspension culture by two and six folds, respectively (
< 0.001). As enhanced insulin response was only observed where both the perfusion and 3D cellular organization were present, this could represent important elements in engineering a functional bioartificial pancreas.
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Details
- Title
- 3D perfusion culture of mouse insulinoma in macro-porous scaffolds enhanced insulin production response
- Creators
- Karn Changsorn - University of TokyoYuan Pang - Tsinghua UniversityHiroaki Matsumoto - Tsinghua UniversityHaofeng Hong - Tsinghua UniversityPierre Wüthrich - University of TokyoWei Sun - Tsinghua UniversityYasuyuki Sakai - University of Tokyo
- Publication Details
- International journal of artificial organs, v 45(1)
- Publisher
- Sage
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000657073400001
- Scopus ID
- 2-s2.0-85098555333
- Other Identifier
- 991019167457604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Transplantation