Journal article
3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation
Biofabrication, v 10(4), pp 044101/1-044101/19
12 Jul 2018
PMID: 29952313
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Human induced pluripotent stem cells (hiPSCs) are more likely to successfully avoid the immunological rejection and ethical problems that are often encountered by human embryonic stem cells in various stem cell studies and applications. To transfer hiPSCs from the laboratory to clinical applications, researchers must obtain sufficient cell numbers. In this study, 3D cell printing was used as a novel method for iPSC scalable expansion. Hydroxypropyl chitin (HPCH), utilized as a new type of bioink, and a set of optimized printing parameters were shown to achieve high cell survival (>90%) after the printing process and high proliferation efficiency (∼32.3 folds) during subsequent 10 d culture. After the culture, high levels of pluripotency maintenance were recognized by both qualitative and quantitative detections. Compared with static suspension culture, hiPSC aggregates formed in 3D-printed constructs showed a higher uniformity in size. Using a novel dual-fluorescent labeling method, hiPSC aggregates in the constructs were found more inclined to form by in situ proliferation rather than multicellular aggregation. This study revealed unique advantages of non-ionic crosslinking bioink material HPCH, including high gel strength and rapid temperature response in hiPSC printing, and achieved primed state hiPSC printing for the first time. Features achieved in this study, such as high cell yield, high pluripotency maintenance and uniform aggregation provide good foundations for further hiPSC studies on 3D micro-tissue differentiation and drug screening.
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Details
- Title
- 3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation
- Creators
- Yang Li - Tsinghua UniversityXulin Jiang - Wuhan UniversityLing Li - Air Force Medical UniversityZhi-Nan Chen - Air Force Medical UniversityGe Gao - Ixcell Biotech Shanghai, Shanghai, People’s Republic of ChinaRui Yao - Tsinghua UniversityWei Sun - Tsinghua University
- Publication Details
- Biofabrication, v 10(4), pp 044101/1-044101/19
- Publisher
- IOP Publishing
- Number of pages
- 19
- Grant note
- Biomanufacturing and Medical Devices Frontier Innovation Center, Department of Mechanical Engineering, Tsinghua University B17026 / Overseas Expertise Introduction Project for Discipline Innovation (111 Project) 31500818; 51235006 / National Natural Science Foundation of China (https://doi.org/10.13039/501100001809) 2015ZX09501009 / National Science and Technology Major Projects for New Drug Development
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000438665600001
- Scopus ID
- 2-s2.0-85055836716
- Other Identifier
- 991019167705104721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials