Journal article
3D printing of vascularized hepatic tissues with a high cell density and heterogeneous microenvironment
Biofabrication, v 15(4), p45004
01 Oct 2023
PMID: 37429291
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Three-dimensional bioprinting has emerged as an appealing approach for creating functional tissues; however, a lack of suitable bioinks with high cell density and printability has greatly limited our ability to print functional tissues. We address this limitation by developing a granular cell aggregate-based biphasic (GCAB) bioink based on densely packed cell aggregates. The GCAB bioink exhibited the desired shear-thinning and shear-recovery properties for extrusion bioprinting and hyperelastic behaviors postprinting for modeling the mechanical characteristics of soft biological tissues. The GCAB bioink displayed a high cell density (∼1.7 × 108 cells cm−3) without compromising viability (∼83%). We printed dense hepatic tissue constructs with enhanced vascularization and metabolic functions by preorganization of GCAB bioink with a defined heterogeneous microenvironment. By simultaneously printing the GCAB bioink and an endothelial cell-laden gelatin bioink, we successfully produced functional hepatic tissues with a high cell density and a perfusable vascular network. The design of the generalizable GCAB bioink opens new avenues to create functional tissues for therapeutic applications.
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Details
- Title
- 3D printing of vascularized hepatic tissues with a high cell density and heterogeneous microenvironment
- Creators
- Yongcong Fang - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaMengke Ji - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaYi Yang - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaYihan Guo - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaRuobin Sun - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaTing Zhang - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of ChinaWei Sun - Drexel UniversityZhuo Xiong - ‘Biomanufacturing and Engineering Living Systems’ Innovation International Talents Base (111 Base) , Beijing 100084, People’s Republic of China
- Publication Details
- Biofabrication, v 15(4), p45004
- Publisher
- IOP Publishing
- Number of pages
- 11
- Grant note
- 2018YFA0703004 / National Key Research and Development Program of China 2021TQ0184 / China Postdoctoral Science Foundation (http://dx.doi.org/10.13039/501100002858) No. U21A20394 / National Natural Science Foundation of China (http://dx.doi.org/10.13039/501100001809) 53330200321 / New Faculty Start-up Funding of Tsinghua University
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:001032730300001
- Scopus ID
- 2-s2.0-85165518999
- Other Identifier
- 991022207061204721
UN Sustainable Development Goals (SDGs)
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials