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Journal article
53BP1 contributes to a robust genomic stability in human fibroblasts
Aging (Albany, NY.), v 3(9), pp 836-845
01 Sep 2011
PMCID: PMC3227449
PMID: 21931182
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Faithful repair of damaged DNA is a crucial process in maintaining cell viability and function. A multitude of factors and pathways guides this process and includes repair proteins and cell cycle checkpoint factors. Differences in the maintenance of genomic processes are one feature that may contribute to species-specific differences in lifespan. We predicted that 53BP1, a key transducer of the DNA damage response and cell cycle checkpoint control, is highly involved in maintaining genomic stability and may function differently in cells from different species. We demonstrate a difference in the levels and recruitment of 53BP1 in mouse and human cells following DNA damage. In addition, we show that unresolved DNA damage persists more in mouse cells than in human cells, as evidenced by increased numbers of micronuclei. The difference in micronuclei seems to be related to the levels of 53BP1 present in cells. Finally, we present evidence that unresolved DNA damage correlates with species lifespan. Taken together, these studies suggest a link between recruitment of 53BP1, resolution of DNA damage, and increased species lifespan.
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Details
- Title
- 53BP1 contributes to a robust genomic stability in human fibroblasts
- Creators
- Lauren S. Fink - Drexel UniversityMichaela Roell - G Moruzzi Univ Bologna, Dept Biochem, Bologna, ItalyEmanuela Caiazza - G Moruzzi Univ Bologna, Dept Biochem, Bologna, ItalyChad Lerner - Drexel Univ, Coll Med, Dept Pathol, Philadelphia, PA 19102 USAThomas Stamato - Lankenau Inst Med Res, Wynnewood, PA 19096 USASilvana Hrelia - G Moruzzi Univ Bologna, Dept Biochem, Bologna, ItalyAntonello Lorenzini - G Moruzzi Univ Bologna, Dept Biochem, Bologna, ItalyChristian Sell - Drexel University
- Publication Details
- Aging (Albany, NY.), v 3(9), pp 836-845
- Publisher
- Impact Journals Llc
- Number of pages
- 10
- Grant note
- R01AG022443 / NATIONAL INSTITUTE ON AGING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) AG022443 / National Institute on Aging; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Aging (NIA) Drexel Aging Initiative
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000296798400005
- Scopus ID
- 2-s2.0-81855209312
- Other Identifier
- 991019168784504721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Geriatrics & Gerontology