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Journal article
A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions
Plasma chemistry and plasma processing, v 38(1), pp 1-12
01 Jan 2018
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A comparative study of two plasma sources (floating-electrode dielectric barrier discharge, DBD, Drexel University; atmospheric pressure argon plasma jet, kINPen, INP Greifswald) on cancer cell toxicity was performed. Cell culture protocols, cytotoxicity assays, and procedures for assessment of hydrogen peroxide (H2O2) were standardized between both labs. The inhibitory concentration 50 (IC50) and its corresponding H2O2 deposition was determined for both devices. For the DBD, IC50 and H2O2 generation were largely dependent on the total energy input but not pulsing frequency, treatment time, or total number of cells. DBD cytotoxicity could not be replicated by addition of H2O2 alone and was inhibited by larger amounts of liquid present during the treatment. Jet plasma toxicity depended on peroxide generation as well as total cell number and amount of liquid. Thus, the amount of liquid present during plasma treatment in vitro is key in attenuating short-lived species or other physical effects from plasmas. These in vitro results suggest a role of liquids in or on tissues during plasma treatment in a clinical setting. Additionally, we provide a platform for correlation between different plasma sources for a predefined cellular response.
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Details
- Title
- A Comparison of Floating-Electrode DBD and kINPen Jet: Plasma Parameters to Achieve Similar Growth Reduction in Colon Cancer Cells Under Standardized Conditions
- Creators
- Sander Bekeschus - Leibniz Institute for Plasma Science and TechnologyAbraham Lin - Nyheim Plasma Institute, Drexel University, Camden, USAAlexander Fridman - Drexel UniversityKristian Wende - Leibniz Institute for Plasma Science and TechnologyKlaus-Dieter Weltmann - Leibniz Institute for Plasma Science and TechnologyVandana Miller - Nyheim Plasma Institute, Drexel University, Camden, USA
- Publication Details
- Plasma chemistry and plasma processing, v 38(1), pp 1-12
- Publisher
- Springer Nature
- Number of pages
- 12
- Grant note
- 03Z22DN11; 03Z22DN12 / German Federal Ministry of Education and Research (BMBF); Federal Ministry of Education & Research (BMBF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology Department Internal Research; Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000419479000001
- Scopus ID
- 2-s2.0-85028844622
- Other Identifier
- 991019169679704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Engineering, Chemical
- Physics, Applied
- Physics, Fluids & Plasmas