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A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network
Journal article   Open access   Peer reviewed

A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

J. Curtis Nickel, Alisa J. Stephens-Shields, J. Richard Landis, Chris Mullins, Adrie van Bokhoven, M. Scott Lucia, Jeffrey P. Henderson, Bhaswati Sen, Jaroslaw E. Krol, Garth D. Ehrlich, …
Journal of clinical medicine, v 8(3), p415
26 Mar 2019
DOI: https://doi.org/10.3390/jcm8030415
PMID: 30917614
Featured in Collection :   UN Sustainable Development Goals @ Drexel
url
https://www.mdpi.com/2077-0383/8/3/415/pdf?version=1553613632View
Published, Version of Record (VoR) Open
url
https://doi.org/10.3390/jcm8030415View
Published, Version of Record (VoR) Open

Abstract

General & Internal Medicine Life Sciences & Biomedicine Medicine, General & Internal Science & Technology
We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction-electrospray ionization-time-of-flight-mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.

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45 citations in Web of Science
45 citations in Scopus

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UN Sustainable Development Goals (SDGs)

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#5 Gender Equality

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Urology & Nephrology
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