Journal article
A Disposable, Self-Contained PCR Chip
Lab on a chip, v 9(4), pp 606-612
21 Feb 2009
PMID: 19190797
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A disposable, self-contained polymerase chain reaction (PCR) chip with on-board stored, just on time releasable, paraffin-passivated, dry reagents is described. During both storage and sample preparation, the paraffin immobilizes and protects the stored reagents. Fluid flow through the reactor leaves the reagents undisturbed. Prior to the amplification step, the chamber is filled with target analyte suspended in water. Upon heating the PCR chamber to the DNA’s denaturation temperature, the paraffin melts and moves out of the way, and the reagents are released and hydrated. To better understand the reagent release process, a scaled up model of the reactor was constructed and the paraffin migration was visualized. Experiments were carried out with a 30 μl reactor demonstrating detectable amplification (with agarose gel electrophoresis) of 10 fg (~200 copies) of lambda DNA template. The in-reactor storage and on-time release of the PCR reagents reduce the number of needed operations and significantly simplify the flow control that would, otherwise, be needed in lab-on-chip devices.
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Details
- Title
- A Disposable, Self-Contained PCR Chip
- Creators
- Jitae Kim - Department of Mechanical Engineering and Applied Mechanics University of Pennsylvania, Philadelphia, PA 19104-6315, USADoyoung Byun - Department of Aerospace and Information Engineering Konkuk University, Seoul, 143-701, Rep. KoreaMichael G Mauk - Department of Mechanical Engineering and Applied Mechanics University of Pennsylvania, Philadelphia, PA 19104-6315, USAHaim H Bau - Department of Mechanical Engineering and Applied Mechanics University of Pennsylvania, Philadelphia, PA 19104-6315, USA
- Publication Details
- Lab on a chip, v 9(4), pp 606-612
- Publisher
- Royal Society of Chemistry
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000263465900014
- Scopus ID
- 2-s2.0-59649108573
- Other Identifier
- 991014878107804721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Chemistry, Multidisciplinary
- Instruments & Instrumentation
- Nanoscience & Nanotechnology