Journal article
A GAL4-Driver Line Resource for Drosophila Neurobiology
Cell reports (Cambridge), v 2(4), pp 991-1001
25 Oct 2012
PMID: 23063364
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
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Details
- Title
- A GAL4-Driver Line Resource for Drosophila Neurobiology
- Creators
- Arnim Jenett - Janelia Research CampusGerald M. Rubin - Janelia Research CampusTeri-T. B. Ngo - Janelia Research CampusDavid Shepherd - Bangor UniversityChristine Murphy - Janelia Research CampusHeather Dionne - Janelia Research CampusBarret D. Pfeiffer - Janelia Research CampusAmanda Cavallaro - Janelia Research CampusDonald Hall - Janelia Research CampusJennifer Jeter - Janelia Research CampusNirmala Iyer - Janelia Research CampusDona Fetter - Janelia Research CampusJoanna H. Hausenfluck - Janelia Research CampusHanchuan Peng - Janelia Research CampusEric T. Trautman - Janelia Research CampusRobert R. Svirskas - Janelia Research CampusEugene W. Myers - Janelia Research CampusZbigniew R. Iwinski - Carl Zeiss (United States)Yoshinori Aso - Janelia Research CampusGina M. DePasquale - Janelia Research CampusAdrianne Enos - Janelia Research CampusPhuson Hulamm - Janelia Research CampusShing Chun Benny Lam - MED InstituteHsing-Hsi Li - Janelia Research CampusTodd R. Laverty - Janelia Research CampusFuhui Long - Janelia Research CampusLei Qu - Janelia Research CampusSean D. Murphy - Janelia Research CampusKonrad Rokicki - Janelia Research CampusTodd Safford - Janelia Research CampusKshiti Shaw - Janelia Research CampusJulie H. Simpson - MED InstituteAllison Sowell - Janelia Research CampusSusana Tae - Janelia Research CampusYang Yu - MED InstituteChristopher T. Zugates - Janelia Research Campus
- Publication Details
- Cell reports (Cambridge), v 2(4), pp 991-1001
- Publisher
- Elsevier
- Number of pages
- 11
- Grant note
- Howard Hughes Medical Institute
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000314455600028
- Scopus ID
- 2-s2.0-84868114222
- Other Identifier
- 991021448030604721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology