Journal article
A GAL80 Collection To Inhibit GAL4 Transgenes in Drosophila Olfactory Sensory Neurons
G3 : genes - genomes - genetics, v 8(11), pp 3661-3668
01 Nov 2018
PMID: 30262521
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Fruit flies recognize hundreds of ecologically relevant odors and respond appropriately to them. The complexity, redundancy and interconnectedness of the olfactory machinery complicate efforts to pinpoint the functional contributions of any component neuron or receptor to behavior. Some contributions can only be elucidated in flies that carry multiple mutations and transgenes, but the production of such flies is currently labor-intensive and time-consuming. Here, we describe a set of transgenic flies that express the Saccharomyces cerevisiae GAL80 in specific olfactory sensory neurons (OrX-GAL80s). The GAL80s effectively and specifically subtract the activities of GAL4-driven transgenes that impart anatomical and physiological phenotypes. OrX-GAL80s can allow researchers to efficiently activate only one or a few types of functional neurons in an otherwise nonfunctional olfactory background. Such experiments will improve our understanding of the mechanistic connections between odorant inputs and behavioral outputs at the resolution of only a few functional neurons.
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Details
- Title
- A GAL80 Collection To Inhibit GAL4 Transgenes in Drosophila Olfactory Sensory Neurons
- Creators
- Jessica Eliason - Janelia Research CampusAli Afify - Johns Hopkins MedicineChristopher Potter - Johns Hopkins MedicineIchiro Matsumura - Emory University
- Publication Details
- G3 : genes - genomes - genetics, v 8(11), pp 3661-3668
- Publisher
- Oxford Univ Press
- Number of pages
- 8
- Grant note
- MCB- 1413062 / National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000449381500025
- Scopus ID
- 2-s2.0-85056281378
- Other Identifier
- 991021229990104721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Genetics & Heredity