A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells

Amber L Jolly; Chi-Hao Luan; Brendon E Dusel; Sara F Dunne; Michael Winding; Vishrut J Dixit; Chloe Robins; Jennifer L Saluk; David J Logan; Anne E Carpenter; Manu Sharma; Deborah Dean; Andrew R Cohen; Vladimir I Gelfand

doi:10.1016/j.celrep.2015.12.051

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A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells

Journal article

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Peer reviewed

A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells

Amber L Jolly, Chi-Hao Luan, Brendon E Dusel, Sara F Dunne, Michael Winding, Vishrut J Dixit, Chloe Robins, Jennifer L Saluk, David J Logan, Anne E Carpenter, …

Cell reports (Cambridge), v 14(3), pp 611-620

26 Jan 2016

DOI: https://doi.org/10.1016/j.celrep.2015.12.051

PMID: 26774481

Featured in Collection : UN Sustainable Development Goals @ Drexel

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Abstract

Animals

Bayes Theorem

Cells, Cultured

Drosophila - genetics

Drosophila - metabolism

Drosophila Proteins - antagonists & inhibitors

Drosophila Proteins - genetics

Drosophila Proteins - metabolism

Dyneins - antagonists & inhibitors

Dyneins - genetics

Dyneins - metabolism

Genome

Lysosomes - physiology

Microtubules - metabolism

Phenotype

rab GTP-Binding Proteins - antagonists & inhibitors

rab GTP-Binding Proteins - genetics

rab GTP-Binding Proteins - metabolism

RNA Interference

RNA, Double-Stranded - metabolism

Time-Lapse Imaging

Long-distance intracellular transport of organelles, mRNA, and proteins ("cargo") occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. We capitalize on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require enzymatically active kinesin-1 motor protein for their formation. We use an automated tracking program and a naive Bayesian classifier for the multivariate motility data to analyze 15,683 gene phenotypes and find 98 proteins involved in regulating lysosome motility along microtubules and 48 involved in the formation of microtubule filled processes in S2 cells. We identify innate immunity genes, ion channels, and signaling proteins having a role in lysosome motility regulation and find an unexpected relationship between the dynein motor, Rab7a, and lysosome motility regulation.

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5 citations in Scopus

Details

Title: A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells
Creators: Amber L Jolly - Children's Hospital Oakland Research Institute
Chi-Hao Luan - High Throughput Analysis Laboratory, Northwestern University, Evanston, IL, 60208, USA.
Brendon E Dusel - High Throughput Analysis Laboratory, Northwestern University, Evanston, IL, 60208, USA.
Sara F Dunne - High Throughput Analysis Laboratory, Northwestern University, Evanston, IL, 60208, USA.
Michael Winding - Northwestern University
Vishrut J Dixit - Northwestern University
Chloe Robins - High Throughput Analysis Laboratory, Northwestern University, Evanston, IL, 60208, USA.
Jennifer L Saluk - High Throughput Analysis Laboratory, Northwestern University, Evanston, IL, 60208, USA.
David J Logan - Broad Institute
Anne E Carpenter - Broad Institute
Manu Sharma - Children's Hospital Oakland Research Institute
Deborah Dean - Children's Hospital Oakland Research Institute
Andrew R Cohen - Drexel University
Vladimir I Gelfand - Northwestern University
Publication Details: Cell reports (Cambridge), v 14(3), pp 611-620
Publisher: Elsevier
Grant note: P30 CA060553 / NCI NIH HHS R01 GM052111 / NIGMS NIH HHS R01 GM089652 / NIGMS NIH HHS
Resource Type: Journal article
Language: English
Academic Unit: Electrical and Computer Engineering
Web of Science ID: WOS:000368701600020
Scopus ID: 2-s2.0-84953337050
Other Identifier: 991019169536104721

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Collaboration types: Domestic collaboration
Web of Science research areas: Cell Biology

A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells

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Abstract

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Details

UN Sustainable Development Goals (SDGs)

InCites Highlights

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