Journal article
A Membrane‐Targeting Aggregation‐Induced Emission Probe for Monitoring Lipid Droplet Dynamics in Ischemia/Reperfusion‐Induced Cardiomyocyte Ferroptosis
Advanced science, v 11(26), 2309907
01 Jul 2024
PMID: 38696589
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Myocardial ischemia/reperfusion injury (MIRI) is the leading cause of irreversible myocardial damage. A pivotal pathogenic factor is ischemia/reperfusion (I/R)‐induced cardiomyocyte ferroptosis, marked by iron overload and lipid peroxidation. However, the impact of lipid droplet (LD) changes on I/R‐induced cardiomyocyte ferroptosis is unclear. In this study, an aggregation‐induced emission probe, TPABTBP is developed that is used for imaging dynamic changes in LD during myocardial I/R‐induced ferroptosis. TPABTBP exhibits excellent LD‐specificity, superior capability for monitoring lipophagy, and remarkable photostability. Molecular dynamics (MD) simulation and super‐resolution fluorescence imaging demonstrate that the TPABTBP is specifically localized to the phospholipid monolayer membrane of LDs. Imaging LDs in cardiomyocytes and myocardial tissue in model mice with MIRI reveals that the LD accumulation level increase in the early reperfusion stage (0–9 h) but decrease in the late reperfusion stage (>24 h) via lipophagy. The inhibition of LD breakdown significantly reduces the lipid peroxidation level in cardiomyocytes. Furthermore, it is demonstrated that chloroquine (CQ), an FDA‐approved autophagy modulator, can inhibit ferroptosis, thereby attenuating MIRI in mice. This study describes the dynamic changes in LD during myocardial ischemia injury and suggests a potential therapeutic target for early MIRI intervention. A a membrane‐targeting aggregation‐induced emission probe is devised to monitor lipid droplet (LD) dynamics during ischemia/reperfusion‐induced cardiomyocyte ferroptosis. These results reveals an increase in LDs during the early reperfusion stage (0–9 h), followed by degradation through lipophagy during the late reperfusion stage (>24 h). Furthermore, inhibiting LD degradation markedly mitigates myocardial ischemia/reperfusion injury in vivo by suppressing ferroptosis.
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Details
- Title
- A Membrane‐Targeting Aggregation‐Induced Emission Probe for Monitoring Lipid Droplet Dynamics in Ischemia/Reperfusion‐Induced Cardiomyocyte Ferroptosis
- Creators
- Yihui Wang - Union HospitalYuan Song - Union HospitalLingling Xu - Union HospitalWuqi Zhou - Union HospitalWenyuan Wang - Union HospitalQiaofeng Jin - Union HospitalYuji Xie - Union HospitalJunmin Zhang - Union HospitalJing Liu - Union HospitalWenqian Wu - Union HospitalHe Li - Union HospitalLe Liang - Wuhan UniversityJing Wang - Union HospitalYali Yang - Union HospitalXiongwen Chen - Tianjin Medical University General HospitalShuping Ge - Drexel UniversityTang Gao - Huazhong University of Science and TechnologyLi Zhang - Union HospitalMingxing Xie (Corresponding Author) - Huazhong University of Science and Technology
- Publication Details
- Advanced science, v 11(26), 2309907
- Publisher
- Wiley
- Grant note
- JCYJ20210324141216040 / Shenzhen Science and Technology 2023AFB753; 2021CFA046 / Natural Science Foundation of Hubei
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Medicine
- Web of Science ID
- WOS:001214572900001
- Scopus ID
- 2-s2.0-85191704749
- Other Identifier
- 991022202102404721
UN Sustainable Development Goals (SDGs)
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Source: SDGs in the Output
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology