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Journal article
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
Journal of visualized experiments, v 2016(111), e53955
13 May 2016
PMID: 27213265
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The new model presented here can be used to understand the influence of hemodynamics on specific cardiac developmental processes, at the cellular and molecular level. To alter intracardiac hemodynamics, fertilized chicken eggs are incubated in a humidified chamber to obtain embryos of the desired stage (HH17). Once this developmental stage is achieved, the embryo is maintained ex ovo and hemodynamics in the embryonic heart are altered by partially constricting the outflow tract (OFT) with a surgical suture at the junction of the OFT and ventricle (OVJ). Control embryos are also cultured ex ovo but are not subjected to the surgical intervention. Banded and control embryos are then incubated in a humidified incubator for the desired period of time, after which 2D ultrasound is employed to analyze the change in blood flow velocity at the OVJ as a result of OFT banding. Once embryos are maintained ex ovo, it is important to ensure adequate hydration in the incubation chamber so as to prevent drying and eventually embryo death. Using this new banded model, it is now possible to perform analyses of changes in the expression of key players involved in valve development and to understand the role of hemodynamics on cellular responses in vivo, which could not be achieved previously.
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Details
- Title
- A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
- Creators
- Vinal Menon - University of South CarolinaLorain Junor - University of South CarolinaMarwa Balhaj - University of South CarolinaJohn F Eberth - University of South CarolinaJay D Potts - University of South Carolina
- Publication Details
- Journal of visualized experiments, v 2016(111), e53955
- Publisher
- JOVE
- Grant note
- P20 RR016434 / NCRR NIH HHS P20 GM103499 / NIGMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000380259900052
- Scopus ID
- 2-s2.0-84971539114
- Other Identifier
- 991021902593304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cardiac & Cardiovascular Systems