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Journal article
A Novel Mass Spectrometry Platform for Multiplexed N-Glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-Glycan Imaging
Analytical chemistry (Washington), v 91(13), pp 8429-8435
02 Jul 2019
PMID: 31177770
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A new platform for N-glycoprotein analysis from serum that combines matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) workflows with antibody slide arrays is described. Antibody panel based (APB) N-glycan imaging allows for the specific capture of N-glycoproteins by antibodies on glass slides and N-glycan analysis in a protein-specific and multiplexed manner. Development of this technique has focused on characterizing two abundant and well-studied human serum glycoproteins, alpha-1-antitrypsin and immunoglobulin G. Using purified standard solutions and 1 μL samples of human serum, both glycoproteins can be immunocaptured and followed by enzymatic release of N-glycans. N-Glycans are detected with a MALDI FT-ICR mass spectrometer in a concentration-dependent manner while maintaining specificity of capture. Importantly, the N-glycans detected via slide-based antibody capture were identical to that of direct analysis of the spotted standards. As a proof of concept, this workflow was applied to patient serum samples from individuals with liver cirrhosis to accurately detect a characteristic increase in an IgG N-glycan. This novel approach to protein-specific N-glycan analysis from an antibody panel can be further expanded to include any glycoprotein for which a validated antibody exists. Additionally, this platform can be adapted for analysis of any biofluid or biological sample that can be analyzed by antibody arrays.
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Details
- Title
- A Novel Mass Spectrometry Platform for Multiplexed N-Glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-Glycan Imaging
- Creators
- Alyson P Black - Medical University of South CarolinaHongyan Liang - Medical University of South CarolinaConnor A West - Medical University of South CarolinaMengjun Wang - Medical University of South CarolinaHarmin P Herrera - Drexel UniversityBrian B Haab - Van Andel InstitutePeggi M Angel - Medical University of South CarolinaRichard R Drake - Medical University of South CarolinaAnand S Mehta - Medical University of South Carolina
- Publication Details
- Analytical chemistry (Washington), v 91(13), pp 8429-8435
- Publisher
- American Chemical Society; Washington, DC
- Grant note
- R21 CA225474 / NCI NIH HHS TL1 TR001451 / NCATS NIH HHS UL1 TR001450 / NCATS NIH HHS U01 CA226052 / NCI NIH HHS U01 CA226158 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Medicine (Graduate)
- Web of Science ID
- WOS:000474477900062
- Scopus ID
- 2-s2.0-85069264938
- Other Identifier
- 991019168287904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical