Journal article
A Novel Method for Enzyme Immobilization: Direct Encapsulation of Acid Phosphatase in Nanoporous Silica Host Materials
Journal of nanoscience and nanotechnology, v 1(1), pp 83-93
01 Mar 2001
PMID: 12914036
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Immobilization of acid phosphatase (ACP) in mesoporous or, more generally, nanoporous silica has been accomplished via the sol-gel reactions of tetramethyl orthosilicate in the presence of ACP and
of d-glucose (DG) as a nonsurfactant template, which is subsequently removed by water extraction after the formation of nanocomposite gels. Characterization of the silica host after the removal of DG shows
that the pore size and volume generally increase with the DG content. At high DG contents, the silica hosts are nanoporous with interconnected nanoscaled pores/channels of regular diameter (e.g., 3.4 nm).
Catalytic activity of ACP encapsulated in nanoporous hosts is significantly improved over that in microporous host prepared in the absence of DG. The apparent enzymatic activity at various pH values and
substrate concentrations correlates well with the nanostructures of the host matrices. As the DG content is increased in the synthesis, the activity tends to increase. At a DG content of 42-60 wt%,
the samples exhibit activities about triple that of the template-free control. These and other results from enzymatic kinetic studies suggest that the increase in the pore size and volume facilitates the
transport of the substrate and product molecules in the host matrices, leading to the observed increase in activity. The thermal stability of ACP is remarkably improved upon immobilization. There is no
detectable leakage of ACP from the host matrices and the biogels are reuseable. This study provides a useful protocol for the development of nanotechnology for various biocatalysts and biosensors.
Metrics
Details
- Title
- A Novel Method for Enzyme Immobilization: Direct Encapsulation of Acid Phosphatase in Nanoporous Silica Host Materials
- Creators
- Yen Wei - Drexel UniversityJigeng Xu - Department of Chemistry, Drexel University, Philadelphia, Pennsylvania 19104, USAQiuwei Feng - Department of Chemistry, Drexel University, Philadelphia, Pennsylvania 19104, USAMuduo Lin - Department of Chemistry, Drexel University, Philadelphia, Pennsylvania 19104, USAHua Dong - Department of Chemistry, Drexel University, Philadelphia, Pennsylvania 19104, USAWan-Jin Zhang - Department of Chemistry, Jilin University, Changchun, 130023, ChinaCe Wang - Department of Chemistry, Jilin University, Changchun, 130023, China
- Publication Details
- Journal of nanoscience and nanotechnology, v 1(1), pp 83-93
- Publisher
- American Scientific Publishers
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Marketing
- Web of Science ID
- WOS:000174291300013
- Scopus ID
- 2-s2.0-0001824363
- Other Identifier
- 991019168523404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Chemistry, Multidisciplinary
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter