Journal article
A PMMA microcapillary quantum dot linked immunosorbent assay (QLISA)
Biosensors & bioelectronics, v 24(12), pp 3467-3474
2009
PMID: 19493670
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The development of a simple and inexpensive quantum dot based immunoassay for detecting myeloperoxidase (MPO) in stool samples is reported (QLISA). The method developed utilizes readily available polymethylmethacrylate (PMMA) microcapillaries as substrates for performing the sandwich assay. High power (80
mW) and low power (10
mW) UV-LEDs were tested for their efficiency in maximizing detection sensitivity in a waveguide illumination or a side illumination mode. The results obtained indicate that both waveguide and side illumination modes can be employed for detecting MPO down to 15
ng/mL, however the high power LED in a side illumination mode improves sensitivity and simplifies the data acquisition process. The protocol and sensor robustness was evaluated with animal stool samples spiked with MPO and the results indicate that the sensitivity of detection is not compromised when used in stool samples. The effect of the ionic strength of the environment on the fluorescence stability of quantum dots was evaluated and found to affect the assay only if long imaging times are employed. Replacing the buffer with glycerol during imaging increased the fluorescence intensity of quantum dots while significantly minimized the loss in intensity even after 2
h.
Metrics
Details
- Title
- A PMMA microcapillary quantum dot linked immunosorbent assay (QLISA)
- Creators
- Sundar Babu - Drexel UniversitySakya Mohapatra - Drexel UniversityLeonid Zubkov - Drexel UniversitySreekant Murthy - Drexel UniversityElisabeth Papazoglou - Drexel University
- Publication Details
- Biosensors & bioelectronics, v 24(12), pp 3467-3474
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000269278300011
- Scopus ID
- 2-s2.0-67650091546
- Other Identifier
- 991019169654004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biophysics
- Biotechnology & Applied Microbiology
- Chemistry, Analytical
- Electrochemistry
- Nanoscience & Nanotechnology