Journal article
A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS)
Sensors (Basel, Switzerland), v 11(5), pp 5520-5528
20 May 2011
PMID: 22413149
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Piezoelectric microcantilever sensors (PEMS) can be sensitive tools for the detection of proteins and cells in biological fluids. However, currently available PEMS can only be used a single time or must be completely stripped and refunctionalized prior to subsequent uses. Here we report the successful use of an alternative regeneration protocol employing high salt concentrations to remove the target, leaving the functional probe immobilized on the microcantilever surface. Our model system employed the extracellular domain (ECD) of recombinant human Epidermal Growth Factor Receptor (EGFR) as the probe and anti-human EGFR polyclonal antibodies as the target. We report that high concentrations of MgCl
2
dissociated polyclonal antibodies specifically bound to EGFR ECD immobilized on the sensor surface without affecting its bioactivity. This simple regeneration protocol both minimized the time required to re-conjugate the probe and preserved the density of probe immobilized on PEMS surface, yielding identical biosensor sensitivity over a series of assays.
Metrics
Details
- Title
- A Rapid Method to Regenerate Piezoelectric Microcantilever Sensors (PEMS)
- Creators
- LiNa Loo - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA; E-MailsWei Wu - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA; E-MailsWan Y Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA; E-MailsWei-Heng Shih - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA; E-MailsHossein Borghaei - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA; E-MailsKambiz Pourrezaei - Department of Materials Science and Engineering, Drexel University, Philadelphia, PA 19104, USA; E-MailsGregory P Adams - Developmental Therapeutics Program, Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111, USA; E-Mails
- Publication Details
- Sensors (Basel, Switzerland), v 11(5), pp 5520-5528
- Publisher
- Molecular Diversity Preservation International (MDPI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering
- Web of Science ID
- WOS:000290947700055
- Scopus ID
- 2-s2.0-79957682347
- Other Identifier
- 991014878615104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Engineering, Electrical & Electronic
- Instruments & Instrumentation