Journal article
A Septin Diffusion Barrier at the Base of the Primary Cilium Maintains Ciliary Membrane Protein Distribution
Science (American Association for the Advancement of Science), v 329(5990), pp 436-439
23 Jul 2010
PMID: 20558667
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In animal cells, the primary cilium transduces extracellular signals through signaling receptors localized in the ciliary membrane, but how these ciliary membrane proteins are retained in the cilium is unknown. We found that ciliary membrane proteins were highly mobile, but their diffusion was impeded at the base of the cilium by a diffusion barrier. Septin 2 (SEPT2), a member of the septin family of guanosine triphosphatases that form a diffusion barrier in budding yeast, localized at the base of the ciliary membrane. SEPT2 depletion resulted in loss of ciliary membrane protein localization and Sonic hedgehog signal transduction, and inhibited ciliogenesis. Thus, SEPT2 is part of a diffusion barrier at the base of the ciliary membrane and is essential for retaining receptor-signaling pathways in the primary cilium.
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Details
- Title
- A Septin Diffusion Barrier at the Base of the Primary Cilium Maintains Ciliary Membrane Protein Distribution
- Creators
- Qicong Hu - Department of Biology, Stanford University, Stanford, CA 94305, USALjiljana Milenkovic - Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USAHua Jin - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USAMatthew P Scott - Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USAMaxence V Nachury - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USAElias T Spiliotis - Department of Biology, Drexel University, Philadelphia, PA 19104, USAW. James Nelson - Department of Biology, Stanford University, Stanford, CA 94305, USA
- Publication Details
- Science (American Association for the Advancement of Science), v 329(5990), pp 436-439
- Publisher
- American Association for the Advancement of Science (AAAS)
- Grant note
- HHMI_SCOTT_M || HHMI_ / Howard Hughes Medical Institute : R37 GM035527-27 || GM / National Institute of General Medical Sciences : NIGMS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000280196500036
- Scopus ID
- 2-s2.0-77954841928
- Other Identifier
- 991014877935704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology