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A Substituted Tetrahydro-Tetrazolo-Pyrimidine Is a Specific and Novel Inhibitor of Hepatitis B Virus Surface Antigen Secretion
Journal article   Open access   Peer reviewed

A Substituted Tetrahydro-Tetrazolo-Pyrimidine Is a Specific and Novel Inhibitor of Hepatitis B Virus Surface Antigen Secretion

Anne Marie Dougherty, Haitao Guo, Gael Westby, Yuanjie Liu, Ender Simsek, Ju-Tao Guo, Anand Mehta, Pamela Norton, Baohua Gu, Timothy Block, …
Antimicrobial agents and chemotherapy, v 51(12), pp 4427-4437
Dec 2007
DOI: https://doi.org/10.1128/AAC.00541-07
PMID: 17875990
Featured in Collection :   UN Sustainable Development Goals @ Drexel
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https://doi.org/10.1128/AAC.00541-07View
Published, Version of Record (VoR) Open

Abstract

Antiviral Agents
The high levels of hepatitis B virus (HBV) surface antigen (HBsAg)-bearing subviral particles in the serum of chronically infected individuals are thought to play a role in suppressing the HBV-specific immune response. Current therapeutics are not directed at reducing this viral antigenemia; thus, our group has focused on identifying inhibitors of HBsAg secretion. By using the HBV-expressing cell line HepG2.2.15, high-throughput screening of an 80,288-compound synthetic small-molecule library identified HBF-0259, an aromatically substituted tetrahydro-tetrazolo-(1, 5- a )-pyrimidine. Following resynthesis, HBF-0259 had a 50% effective concentration of approximately 1.5 μM in a secondary, HBV-expressing cell line, with a concentration that exhibited 50% cytotoxicity of >50 μM. The equilibrium concentration of HBF-0259 in aqueous solution at physiological pH was 15 to 16 μM; the selective index was thus >9. As intended by our screening paradigm, HBF-0259 is a selective, potent inhibitor of secretion of both subviral and DNA-containing viral particles, while the secretion of α-1-acid glycoprotein and α-1-antitrypsin was unaffected. The HBV e antigen, which is not a constituent of HBV particles, was also unaffected, suggesting that the secretion of particles bearing HBV structural glycoproteins is targeted directly. Inhibitory activity was also confirmed by transfection of HBsAg, indicating that the action of the compound is independent of those of other viral proteins. HBF-0259 had no effect on HBV DNA synthesis, demonstrating that inhibition is independent of viral genomic replication. Finally, HBF-0259 had little or no effect on the cell-to-cell spread of two unrelated viruses, suggesting that it is a specific inhibitor of secretion of HBsAg. Possible mechanisms of action and the implications for its development are discussed.

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Web of Science research areas
Microbiology
Pharmacology & Pharmacy
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