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Journal article
A Three-Dimensional In Vitro Coculture Model to Quantify Breast Epithelial Cell Adhesion to Endothelial Cells
Tissue engineering. Part C, Methods, v 25(10), pp 69-618
01 Oct 2019
PMID: 31441384
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Three-dimensional (3D)
in vitro
culture models better recapitulate the tissue microenvironment, and therefore may provide a better platform to evaluate therapeutic effects on adhesive cell–cell interactions. The objective of this study was to determine if AD-01, a peptide derivative of FK506-binding protein like that is reported to bind to the adhesion receptor CD44, would induce a greater reduction in breast epithelial spheroid adhesion to endothelial tube-like networks in our 3D coculture model system compared to two-dimensional (2D) culture. MCF10A, MCF10A-NeuN, MDA-MB-231, and MCF7 breast epithelial cells were pretreated with AD-01 either as single cells or as spheroids. Breast epithelial cell adhesion to 2D tissue culture substrates was first measured, followed by spheroid formation (breast cell–cell adhesion) and spheroid adhesion to Matrigel or endothelial networks. Finally,
CD44
expression was quantified in breast epithelial cells in 2D and 3D culture. Our results show that AD-01 had the largest effect on spheroid formation, specifically in breast cancer cell lines. AD-01 also inhibited breast cancer spheroid adhesion to and migration along endothelial networks. The different breast epithelial cell lines expressed more CD44 when cultured as 3D spheroids, but this did not universally translate into higher protein levels. This study shows that 3D coculture models can enable unique insights into cell adhesion, migration, and cell–cell interactions, thereby enhancing understanding of basic biological mechanisms. Furthermore, such 3D coculture systems may also represent a more relevant testing platform for understanding the mechanism-of-action of new therapeutic agents.
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Details
- Title
- A Three-Dimensional In Vitro Coculture Model to Quantify Breast Epithelial Cell Adhesion to Endothelial Cells
- Creators
- Swathi Swaminathan - Drexel UniversityAaron N. Cranston - Precision TherapeuticsAlisa Morss Clyne - University of Maryland, College Park
- Publication Details
- Tissue engineering. Part C, Methods, v 25(10), pp 69-618
- Publisher
- Mary Ann Liebert, Inc., publishers
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000488129700001
- Scopus ID
- 2-s2.0-85073182964
- Other Identifier
- 991019167449504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell & Tissue Engineering
- Cell Biology
- Engineering, Biomedical
- Materials Science, Biomaterials