Journal article
A ceramide-activated protein phosphatase mediates ceramide-induced G(1) arrest of Saccharomyces cerevisiae
Genes & development, v 10(4), pp 382-394
15 Feb 1996
PMID: 8600023
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Abstract
Certain mammalian growth modulators, such as tumor necrosis factor alpha, interleukin-1 beta, and gamma-interferon, induce an antiproliferative response-terminal differentiation, apoptosisis, or cell cycle arrest-through a novel signal transduction pathway mediated by the lipid ceramide as a second messenger. Both a ceramide-activated protein phosphatase and a ceramide-activated protein kinase have been implicated in transmitting the signals elicited by ceramide. We have determined that ceramide addition to the yeast Saccharomyces causes a similar antiproliferative response, resulting in arrest of cells in the G(1) phase of the cell cycle. We have also determined that yeast cells contain a ceramide-activated protein phosphatase composed of regulatory subunits encoded by TPD3 and CDC55 and a catalytic subunit encoded by SIT4. Because mutation of any one of these three genes renders strains resistant to ceramide inhibition, we conclude that the G(1) effects of ceramide are mediated at least in part by the yeast ceramide-activated protein phosphatase. These results highlight the conservation of signaling systems in yeast and mammalian cells and provide a novel approach to dissecting this ubiquitous signal transduction pathway.
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Details
- Title
- A ceramide-activated protein phosphatase mediates ceramide-induced G(1) arrest of Saccharomyces cerevisiae
- Creators
- J T NickelsBroach
- Publication Details
- Genes & development, v 10(4), pp 382-394
- Publisher
- COLD SPRING HARBOR LAB PRESS
- Number of pages
- 13
- Grant note
- P01CA041086 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) CA41086 / NCI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1996TY96500002
- Scopus ID
- 2-s2.0-0029915925
- Other Identifier
- 991021229986004721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology
- Developmental Biology
- Genetics & Heredity