Journal article
A combined transcriptome and proteome survey of malaria parasite liver stages
Proceedings of the National Academy of Sciences - PNAS, v 105(1), pp 305-310
08 Jan 2008
PMID: 18172196
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
For 50 years since their discovery, the malaria parasite liver stages (LS) have been difficult to analyze, impeding their utilization as a critical target for antiinfection vaccines and drugs. We have undertaken a comprehensive transcriptome analysis in combination with a proteomic survey of LS. Green fluorescent protein-tagged
Plasmodium yoelii
(PyGFP) was used to efficiently isolate LS-infected hepatocytes from the rodent host. Genome-wide LS gene expression was profiled and compared with other parasite life cycle stages. The analysis revealed ≈2,000 genes active during LS development, and proteomic analysis identified 816 proteins. A subset of proteins appeared to be expressed in LS only. The data revealed exported parasite proteins and LS metabolic pathways including expression of FASII pathway enzymes. The FASII inhibitor hexachlorophene and the antibiotics, tetracycline and rifampicin, that target the apicoplast inhibited LS development, identifying FASII and other pathways localized in the apicoplast as potential drug targets to prevent malaria infection.
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Details
- Title
- A combined transcriptome and proteome survey of malaria parasite liver stages
- Creators
- Alice S Tarun - Seattle Biomedical Research Institute, Seattle, WA 98109Xinxia Peng - Seattle Biomedical Research Institute, Seattle, WA 98109Ronald F Dumpit - Seattle Biomedical Research Institute, Seattle, WA 98109Yuko Ogata - Seattle Biomedical Research Institute, Seattle, WA 98109Hilda Silva-Rivera - Seattle Biomedical Research Institute, Seattle, WA 98109Nelly Camargo - Seattle Biomedical Research Institute, Seattle, WA 98109Thomas M Daly - Drexel University College of Medicine, Philadelphia, PA 19129; andLawrence W Bergman - Drexel University College of Medicine, Philadelphia, PA 19129; andStefan H. I Kappe - Seattle Biomedical Research Institute, Seattle, WA 98109
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, v 105(1), pp 305-310
- Publisher
- National Academy of Sciences
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; [Retired Faculty]
- Web of Science ID
- WOS:000252435300057
- Scopus ID
- 2-s2.0-38349159563
- Other Identifier
- 991014877710604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology