Journal article
A comparison of techniques for introducing macromolecules into living cells
Cytometry (New York, N.Y.), v 14(3)
1993
PMID: 7682492
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Flow cytometry has been used to evaluate several techniques for introducing macromolecules into large numbers of living cells. One technique is cell fusion with red blood cell ghosts loaded with a fluorescent reporter molecule (RBCF). The second technique, termed osmotic lysis of pinosomes (OLP), involves a brief exposure of cells to a hypertonic solution containing the reporter molecule; subsequently, a hypotonic media is added which lyses the pinosomes formed during the hypertonic treatment. A third technique, scrape loading (SL), involves the creation of transient holes in the cell membrane through the application of mechanical forces, which allows for the passage of reporter molecules into cells. A comparison of these techniques is presented here. OLP appears to offer several advantages: It is a simple procedure, virtually all cells are fluorescently labelled, and it is capable of loading larger amounts of material more uniformly into cells while maintaining excellent viability. © 1993 Wiley‐Liss, Inc.
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Details
- Title
- A comparison of techniques for introducing macromolecules into living cells
- Creators
- Grace Lee - Columbia UniversityThomas M. Delohery - Columbia UniversityZeev RonaiPaul W. Brandt‐RaufMatthew R. Pincus - Syracuse UniversityRandall B. Murphy - New York UniversityI. Bernard Weinstein - Columbia University
- Publication Details
- Cytometry (New York, N.Y.), v 14(3)
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- Number of pages
- 6
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:A1993KR65900004
- Scopus ID
- 2-s2.0-0027456243
- Other Identifier
- 991019323782604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Cell Biology