Journal article
A comparison of two DNA probes, one specific for Plasmodium falciparum and one with wider reactivity, in the diagnosis of malaria
Transactions of the Royal Society of Tropical Medicine and Hygiene, v 84(2)
1990
PMID: 2202099
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The sensitivity and specificity of 2 probes for the detection of malarial infection was studied. 399 blood samples from Gambian children were tested in a deoxyribonucleic acid (DNA) hybridization assay, and the results compared with the microscopical findings from thick blood films. 8 additional pure
Plasmodium malariae and 14 pure
P. vivax samples were also assayed. One probe, containing a 21 base pair tandem repeat and highly specific for
P. falciparum, detected this species in all except 2 of 74 samples with a parasitaemia of 250 per μl or more; the overall sensitivity of the probe was 76%. The other probe, a 6 kilobase pair organelle DNA, is conserved in all
Plasmodium species so far tested. Its sensitivity for
P. falciparum was lower than the 21 base pair repeat, but it detected
P. vivax and
P. malariae at low levels of parasitaemia, and thus could be useful in field studies.
Metrics
Details
- Title
- A comparison of two DNA probes, one specific for Plasmodium falciparum and one with wider reactivity, in the diagnosis of malaria
- Creators
- M Holmberg - Department of Immunology, Karolinska Institute, Stockholm, SwedenA.B Vaidya - Department of Microbiology and Immunology, Hahnemann University, Philadelphia, Pennsylvania, USAF.C Shenton - Medical Research Council Laboratories, Fajara, GambiaR.W Snow - Medical Research Council Laboratories, Fajara, GambiaB.M Greenwood - Medical Research Council Laboratories, Fajara, GambiaH Wigzell - Department of Immunology, Karolinska Institute, Stockholm, SwedenU Pettersson - Department of Medical Genetics, Uppsala University, Uppsala, Sweden
- Publication Details
- Transactions of the Royal Society of Tropical Medicine and Hygiene, v 84(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1990DE87200007
- Scopus ID
- 2-s2.0-0025311636
- Other Identifier
- 991014877975204721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine