Journal article
A conserved DNA structural control element modulates transcription of a mammalian gene
Nucleic acids research, v 20(24), pp 6583-6587
25 Dec 1992
PMID: 1480478
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The mammalian dihydrofolate reductase (DHFR) gene promoters contain several conserved sequence elements which bind protein, and yet there are other conserved DNA sequences that do not footprint. We report here that mutation of one of these conserved non-footprinting regions increases transcription from this promoter both in vitro and in vivo. We show that this conserved region is flanked by sites hypersensitive to cleavage by methidiumpropyl-EDTA-Fe(II). Furthermore, multimers of a double-stranded oligonucleotide comprised of this region display faster migration through polyacrylamide than control DNA. The difterence in mobility is not the result of bending, nor does the primary sequence contain features that would predict altered mobility. We propose that this ‘Structural Control Element’ is rigid and down-regulates transcription by inhibiting interactions between proteins binding adjacent to this region.
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Details
- Title
- A conserved DNA structural control element modulates transcription of a mammalian gene
- Creators
- Andrew J. Pierce - University of North Carolina at Chapel HillRobert C. Jambou - University of North Carolina at Chapel HillDavid E. Jensen - University of North Carolina at Chapel HillJane Clifford Azizkhan - University of North Carolina at Chapel Hill
- Publication Details
- Nucleic acids research, v 20(24), pp 6583-6587
- Publisher
- Oxford University Press
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1992KG70500020
- Scopus ID
- 2-s2.0-0027105002
- Other Identifier
- 991020200754504721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemistry & Molecular Biology