Journal article
A conserved OmpA-like protein in Legionella pneumophila required for efficient intracellular replication
FEMS microbiology letters, v 363(16), pfnw173
01 Aug 2016
PMID: 27421957
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The OmpA-like protein domain has been associated with peptidoglycan-binding proteins, and is often found in virulence factors of bacterial pathogens. The intracellular pathogen Legionella pneumophila encodes for six proteins that contain the OmpA-like domain, among them the highly conserved uncharacterized protein we named CmpA. Here we set out to characterize the CmpA protein and determine its contribution to intracellular survival of L. pneumophila. Secondary structure analysis suggests that CmpA is an inner membrane protein with a peptidoglycan-binding domain at the C-teminus. A cmpA mutant was able to replicate normally in broth, but failed to compete with an isogenic wild-type strain in an intracellular growth competition assay. The cmpA mutant also displayed significant intracellular growth defects in both the protozoan host Acanthamoeba castellanii and in primary bone marrow-derived macrophages, where uptake into the cells was also impaired. The cmpA phenotypes were completely restored upon expression of CmpA in trans. The data presented here establish CmpA as a novel virulence factor of L. pneumophila that is required for efficient intracellular replication in both mammalian and protozoan hosts.
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Details
- Title
- A conserved OmpA-like protein in Legionella pneumophila required for efficient intracellular replication
- Creators
- Ian P. Goodwin - Drexel UniversityOgan K. Kumova - Drexel UniversityShira Ninio - Drexel UniversityMichelle J Dolinski - Physics
- Publication Details
- FEMS microbiology letters, v 363(16), pfnw173
- Publisher
- Oxford Univ Press
- Number of pages
- 9
- Grant note
- Drexel Graduate Student Association Pennsylvania Department of Health
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000380762500010
- Scopus ID
- 2-s2.0-84982943823
- Other Identifier
- 991019186805904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology